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ICOS Antibody (YA6605)

(Synonyms: Activation inducible lymphocyte immunomediatory molecule antibody; Activation-inducible lymphocyte immunomediatory molecule antibody; AILIM antibody; CD278 antibody; CD278 antigen antibody; CD28-related Protein-1 antibody; CRP1 antibody; CVID1 antibody; ICOS antibody; ICOS_HUMAN antibody; Activation inducible lymphocyte immunomediatory molecule antibody; Activation-inducible lymphocyte immunomediatory molecule antibody; AILIM antibody; CD278 antibody; CD278 antigen antibody; CD28-related Protein-1 antibody; CRP1 antibody; CVID1 antibody; ICOS antibody; ICOS_HUMAN antibody; Inducible costimulator antibody; Inducible T cell co stimulator antibody; Inducible T-cell costimulator antibody; MGC39850 antibody; )
Customer Review

Based on 1 Customer Validation

ICOS Antibody (YA6605) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ICOS.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ICOS Antibody (YA6605)
  • ICOS Antibody (YA6605) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000

Product Details

Description

ICOS Antibody (YA6605) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ICOS.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 23 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 23 kDa
Immunogen

Recombinant protein within Human ICOS aa 1-100 / 199.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ICOS Antibody (YA6605)
    Western blot analysis of extracts from Mouse thymus (lane 1(20μg)) 、Mouse spleen (lane 2(20μg)) 、Rat thymus (lane 3(20μg)) and Rat spleen (lane 4(20μg)) using ICOS Antibody (HY-P86912) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    ICOS is a Stimulatory receptor expressed in activated or antigen-experienced T-cells that plays an important role in the immune response. Upon binding to its ligand ICOSL expressed on antigen presenting cells (APCs), delivers costimulatory signals that enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines including IL10, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Also acts as a costimulatory receptor critical for the differentiation of T follicular regulatory cells upon immune challenges such as viral infection. Mechanistically, potentiates TCR-induced calcium flux by augmenting PLCG1 activation and actin remodeling. In addition, activates PI3K signaling pathways independently of calcium flux. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes[1][2][3][4].

  • Subcellular Localization

    Cell membrane,Secreted

  • Expression


    Tissue_Specificity: Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B-cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus
    Induction: By phorbol myristate acetate (PMA) and ionomycin. Up-regulated early on T-cells and continues to be expressed into the later phases of T-cell activation

  • Isoforms & Post-Translational Modification

    Q9Y6W8 has two isomers: Q9Y6W8-1: 22625 Da (predicted); Q9Y6W8-2: 19127 Da (predicted).
    N-glycosylated

  • Subunit

    Homodimer; disulfide-linked

  • SwissProt ID

    Q9Y6W8

  • Gene ID
    29851 [NCBI]
  • Synonyms

    Activation inducible lymphocyte immunomediatory molecule antibody; Activation-inducible lymphocyte immunomediatory molecule antibody; AILIM antibody; CD278 antibody; CD278 antigen antibody; CD28-related Protein-1 antibody; CRP1 antibody; CVID1 antibody; ICOS antibody; ICOS_HUMAN antibody; Activation inducible lymphocyte immunomediatory molecule antibody; Activation-inducible lymphocyte immunomediatory molecule antibody; AILIM antibody; CD278 antibody; CD278 antigen antibody; CD28-related Protein-1 antibody; CRP1 antibody; CVID1 antibody; ICOS antibody; ICOS_HUMAN antibody; Inducible costimulator antibody; Inducible T cell co stimulator antibody; Inducible T-cell costimulator antibody; MGC39850 antibody;

ICOS Antibody (YA6605) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Mouse thymus (lane 1(20μg)) 、Mouse spleen (lane 2(20μg)) 、Rat thymus (lane 3(20μg)) and Rat spleen (lane 4(20μg)) using ICOS Antibody (HY-P86912) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • ICOS Antibody (YA6605) Product Image
    1/16
  • Experimental Validation Results for ICOS Antibody (YA6605)
  • ICOS Antibody (YA6605) Product Image
  • Experimental Validation Results for ICOS Antibody (YA6605)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Mouse thymus (lane 1(20μg)) 、Mouse spleen (lane 2(20μg)) 、Rat thymus (lane 3(20μg)) and Rat spleen (lane 4(20μg)) using ICOS Antibody (HY-P86912) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ICOS Antibody (YA6605)
  • ICOS Antibody (YA6605) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Mouse thymus (lane 1(20μg)) 、Mouse spleen (lane 2(20μg)) 、Rat thymus (lane 3(20μg)) and Rat spleen (lane 4(20μg)) using ICOS Antibody (HY-P86912) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • ICOS Antibody (YA6605) Product Image WB