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CRMP5 Antibody (YA2497)

(Synonyms: CRAM; DPYSL5; DRP5; Ulip6)
Customer Review

Based on 1 Customer Validation

CRMP5 Antibody (YA2497) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CRMP5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CRMP5 Antibody (YA2497)
  • CRMP5 Antibody (YA2497) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:2000 1:100-1:200 1:50

Product Details

Description

CRMP5 Antibody (YA2497) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CRMP5.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 61 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 61 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human CRMP5 aa500-564/564.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CRMP5 Antibody (YA2497)
    Western blot analysis of extracts from Mouse brain tissue (lane2, 20μg), Mouse testis tissue (lane3, 20μg) and Rat brain tissue (lane4, 20μg) using CRMP5 Antibody (HY-P82752). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    CRMP5 is involved in the negative regulation of dendrite outgrowth

  • Subcellular Localization

    Cytoplasm

  • Subunit

    Homotetramer, and heterotetramer with other DPYS-like proteins. Interacts with MAP2 and TUBB3 (PubMed:33894126)

  • SwissProt ID

    Q9BPU6

  • Gene ID
    56896 [NCBI]
  • Synonyms

    CRAM; DPYSL5; DRP5; Ulip6

  • Research Field

    Neuroscience

CRMP5 Antibody (YA2497) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Mouse brain tissue (lane2, 20μg), Mouse testis tissue (lane3, 20μg) and Rat brain tissue (lane4, 20μg) using CRMP5 Antibody (HY-P82752). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • CRMP5 Antibody (YA2497) Product Image
    1/16
  • Experimental Validation Results for CRMP5 Antibody (YA2497)
  • CRMP5 Antibody (YA2497) Product Image
  • Experimental Validation Results for CRMP5 Antibody (YA2497)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Mouse brain tissue (lane2, 20μg), Mouse testis tissue (lane3, 20μg) and Rat brain tissue (lane4, 20μg) using CRMP5 Antibody (HY-P82752). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CRMP5 Antibody (YA2497)
  • CRMP5 Antibody (YA2497) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Mouse brain tissue (lane2, 20μg), Mouse testis tissue (lane3, 20μg) and Rat brain tissue (lane4, 20μg) using CRMP5 Antibody (HY-P82752). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • CRMP5 Antibody (YA2497) Product Image WB