eIF4G1 Antibody

Cat. No.: HY-P80652: Data Sheet COA
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User Guide for Antibodies
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eIF4G1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to eIF4G1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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eIF4G1 Antibody Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation
References

Description

eIF4G1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to eIF4G1.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 175 kDa;

Observed band size: 220 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q04637

Gene ID

1981 [NCBI]

Immunogen

Synthetic peptide corresponding to Human eIF4G.AA range:.1230-1234.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ICC

Immunocytochemistry analysis of C6 cells labeling eIF4G1 with eIF4G1 Antibody (HY-P80652) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with eIF4G1 Antibody ((HY-P80652) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of Hela cells labeling eIF4G1 with eIF4G1 Antibody (HY-P80652) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with eIF4G1 Antibody ((HY-P80652) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome (PubMed:29987188). Exists in two complexes, either with EIF1 or with EIF4E (mutually exclusive) (PubMed:29987188). Together with EIF1, is required for leaky scanning, in particular for avoiding cap-proximal start codon (PubMed:29987188). Together with EIF4E, antagonizes the scanning promoted by EIF1-EIF4G1 and locates the start codon (through a TISU element) without scanning (PubMed:29987188). As a member of the eIF4F complex, required for endoplasmic reticulum stress-induced ATF4 mRNA translation (PubMed:29062139)

Subcellular Localization：Cytoplasm; Nucleus; Cytoplasm, Stress granule

Isoforms & Post-Translational Modification：Q04637 has 8 isomers: Q04637-1: 175491 Da (predicted); Q04637-3: 171514 Da (predicted); Q04637-4: 166619 Da (predicted); Q04637-5: 158517 Da (predicted); Q04637-6: 154805 Da (predicted); Q04637-7: 154933 Da (predicted); Q04637-8: 175619 Da (predicted); Q04637-9: 176237 Da (predicted).
Phosphorylated at multiple sites in vivo. Phosphorylation at Ser-1185 by PRKCA induces binding to MKNK1;Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription

Subunit：eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E (cap-binding) and EIF4G1/EIF4G3 (PubMed:7651417, PubMed:7935836, PubMed:9372926). Interacts with eIF3 complex, mutually exclusive with EIF4A1 or EIF4A2, EIF4E and through its N-terminus with PABPC1 (PubMed:10970864, PubMed:10996799, PubMed:12086624, PubMed:16698552, PubMed:7651417, PubMed:7935836, PubMed:9372926, PubMed:9857202). Interacts with EIF4E or with EIF1 (mutually exclusive) through a common binding site (PubMed:29987188). Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2 (PubMed:11154262, PubMed:9878069). Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E (PubMed:11154262, PubMed:9878069). Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation (PubMed:8521827). EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA (PubMed:10970864, PubMed:10996799, PubMed:12086624, PubMed:9857202). Interacts with EIF4E3 (By similarity). Interacts with CIRBP and MIF4GD (PubMed:10970864, PubMed:10996799, PubMed:16513844, PubMed:18025107). Interacts with RBM4 (PubMed:17284590). Interacts with HNRNPD/AUF1; the interaction requires RNA (PubMed:24423872). Interacts with DDX3X; the interaction requires RNA (PubMed:22872150). Interacts with DAZAP2 (PubMed:17984221)

RRID

AB_3102155

Database

Entrez Gene: 1981 Human

SwissProt: Q04637 Human

OMIM: 614251 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

p220

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/ekvx-cell.html [Content Brief]

eIF4G1 Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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eIF4G1 Antibody

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Background

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eIF4G1 Antibody Related Classifications

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