KIF4A Antibody (YA2653)
(Synonyms: Chromokinesin; KIF4; KIF4 G1; KIF4A; KIF4G1)KIF4A Antibody (YA2653) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KIF4A.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, FC
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Reactivity :
Human, Mouse
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Formulation:
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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FC
FC: Flow Cytometry
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|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 |
Product Details
KIF4A Antibody (YA2653) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KIF4A.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 150 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 140 kDa
Entrez Gene: 24137 Human ; 16571 Mouse ; 84393 Rat
SwissProt: O95239 Human ; P33174 Mouse ;
OMIM: 300923 Human
A synthesized peptide derived from human KIF4A aa40-70/1232.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
KIF4A is an Iron-sulfur (Fe-S) cluster binding motor protein that has a role in chromosome segregation during mitosis. Translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization[1][2][3].
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Subcellular Localization
Nucleus matrix; Cytoplasm; Cytoplasm, cytoskeleton, spindle; Midbody; Chromosome
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Expression
Tissue_specificity:Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung -
Isoforms & Post-Translational Modification
O95239 has 2 isomers: O95239-1: 139881 Da (predicted); O95239-2: 128459 Da (predicted).
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Subunit
Interacts with the cytosolic iron-sulfur protein assembly (CIA) complex components CIAO2B and MMS19; the interactions facilitate the transfer of Fe-S clusters to KIF4A to ensure proper localization of KIF4A to mitotic machinery components (PubMed:29848660). Interacts (via C-terminus) with unphosphorylated PRC1 (via N-terminus); the interaction is required for the progression of mitosis (PubMed:15297875, PubMed:15625105, PubMed:29848660)
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SwissProt ID
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Synonyms
Chromokinesin; KIF4; KIF4 G1; KIF4A; KIF4G1
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Research Field
Signal Transduction
Documentation
References
[1]. Ben-Shimon L, et al. Fe-S cluster coordination of the chromokinesin KIF4A alters its subcellular localization during mitosis. J Cell Sci. 2018 Jun 27;131(12):. [Content Brief]
[2]. Kurasawa Y, et al. Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation. EMBO J. 2004 Aug 18;23(16):3237-48. [Content Brief]
[3]. Zhu C, et al. Cell cycle-dependent translocation of PRC1 on the spindle by Kif4 is essential for midzone formation and cytokinesis. Proc Natl Acad Sci U S A. 2005 Jan 11;102(2):343-8. [Content Brief]
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