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  5. Hck Antibody (YA2963)

Hck Antibody (YA2963) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Hck.

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사이즈 가격 재고 수량
20 μL 해외재고보유
50 μL 해외재고보유
100 μL 해외재고보유
250 μL   견적 받기  
or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • 제품 설명

  • References

제품 설명

Hck Antibody (YA2963) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Hck.
Host

Rabbit
Clonality

Recombinant,Monoclonal
분자량
Predicted band size: 60 kDa;
Observed band size: 60 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthetic peptide of human Hck
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:2000-1:5000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:100
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
선적

Shipping with blue ice.
Background
Function：Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS
Subcellular Localization：Lysosome; Membrane; Lipid-anchor; Cell projection, podosome membrane; Lipid-anchor; Cytoplasm, cytosol; Cell membrane; Lipid-anchor; Membrane, caveola; Lipid-anchor; Cell junction, focal adhesion; Cytoplasm, cytoskeleton; Golgi apparatus; Cytoplasmic vesicle; Lysosome; Nucleus; Cytoplasmic vesicle, secretory vesicle; Cytoplasm, cytosol
Expression：
Tissue_specificity:Detected in monocytes and neutrophils (protein levels) . Primarily expressed in myeloid and B lymphoid cells. Highly expressed in granulocytes. Detected in tonsils.

Induction:Up-regulated during myeloid cell differentiation. The highest levels are detected in fully differentiated phagocytes. Up-regulated by IL2
Isoforms & Post-Translational Modification：P08631 has 4 isomers: P08631-1: 59600 Da (predicted); P08631-2: 57312 Da (predicted); P08631-3: 57241 Da (predicted); P08631-4: 59529 Da (predicted).
Phosphorylated on several tyrosine residues. Autophosphorylated. Becomes rapidly phosphorylated upon activation of the immunoglobulin receptors FCGR1A and FCGR2A. Phosphorylation by the BCR-ABL fusion protein mediates activation of HCK. Phosphorylation at Tyr-411 increases kinase activity. Phosphorylation at Tyr-522 inhibits kinase activity. Kinase activity is not required for phosphorylation at Tyr-522, suggesting that this site is a target of other kinases;Ubiquitinated by CBL, leading to its degradation via the proteasome;Isoform 2 palmitoylation at position 2 requires prior myristoylation. Palmitoylation at position 3 is required for caveolar localization of isoform 2
Subunit：Interacts (via SH2 domain) with FLT3 (tyrosine phosphorylated). Interacts with VAV1, WAS and RAPGEF1 (By similarity). This interaction stimulates its tyrosine-kinase activity. Interacts with ARRB1 and ARRB2. Interacts with ADAM15. Interacts with FASLG. Interacts with CBL. Interacts with FCGR1A; the interaction may be indirect. Interacts with IL6ST. Interacts (via SH3 domain) with ELMO1. Interacts (via SH3 domain) with TP73. Interacts with YAP1. Interacts with ABL1 and ITGB1, and thereby recruits ABL1 to activated ITGB1. Interacts (via SH3 domain) with WDCP
Database

Entrez Gene: 3055 Human

SwissProt: P08631 Human

OMIM: 620296 Human

Research Field

Signal Transduction
Synonyms
HCK; Tyrosine-protein kinase HCK; Hematopoietic cell kinase; Hemopoietic cell kinase; p59-HCK/p60-HCK; p59Hck; p61Hck
각종 서류
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)
References

Hck Antibody (YA2963) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:
Hck Antibody (YA2963)
Cat. No.:
HY-P83218
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SAFETY DATA SHEET (SDS)

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