TORC3 Antibody (YA5086)

Cat. No.: HY-P85394: Ficha de datos User Guide for Antibodies
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TORC3 Antibody (YA5086) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to TORC3.

Para uso exclusivo en investigación. No vendemos a pacientes.

Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

TORC3 Antibody (YA5086) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to TORC3.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Peso molecular

Predicted band size: 67 kDa;

Observed band size: 75 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q6UUV7

Gene ID

64784 [NCBI]

Immunogen

A synthesized peptide derived from human CRTC3. The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-200
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-200

Sensitivity	Endogenous	Pureza	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR)

Subcellular Localization：Nucleus; Cytoplasm

Expression：
Tissue_specificity:It is primarily expressed in B cells and T cells. The highest expression level is found in lung tissue. It is also expressed in the brain, colon, heart, kidneys, ovaries, and prostate. Expression is weaker in the liver, pancreas, muscles, small intestine, spleen, and stomach.

Isoforms & Post-Translational Modification：Q6UUV7 has 2 isomers: Q6UUV7-1: 66959 Da (predicted); Q6UUV7-3: 66830 Da (predicted).
Phosphorylation/dephosphorylation states of Ser-273 are required for regulating transduction of CREB activity (By similarity). CRTCs/TORCs are inactive when phosphorylated, and active when dephosphorylated at this site (By similarity). May be phosphorylated at Ser-391 by MAPK3/ERK1 and/or MAPK1/ERK2 or by some cyclin-dependent kinases such as CDK1,CDK2 or CDK5 (PubMed:30611118). Following adenylyl cyclase activation, dephosphorylated at Ser-162 and Ser-273 resulting in its dissociation from 14-3-3 proteins probably promoting CRTC3 translocation into the nucleus (PubMed:30611118)

Subunit：Binding, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1 enhances recruitment of TAF4 to the promoter (PubMed:14506290). 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction (PubMed:14506290). Interacts (when phosphorylated at Ser-162 and Se-273) with 14-3-3 proteins (PubMed:30611118). Interacts with YWHAE (PubMed:30611118). Interacts (when phosphorylated at Ser-391) with phosphatase PP2A catalytic subunit PPP2CA and regulatory subunits PPP2R1A and PPP2R2A (PubMed:30611118). Interacts, via the N-terminal with the ankyrin repeats of BCL3, to form a complex with CREB1 on CRE and TxRE responsive elements and represses HTLV-1 LTR-mediated transcription (PubMed:17644518)

Synonyms

CRTC3; TORC3; CREB-regulated transcription coactivator 3; Transducer of regulated cAMP response element-binding protein 3; TORC-3; Transducer of CREB protein 3

Documentación

User Guide for Antibodies (1077 KB)