TORC3 Antibody (YA5086)
(Synonyms: CRTC3; TORC3; CREB-regulated transcription coactivator 3; Transducer of regulated cAMP response element-binding protein 3; TORC-3; Transducer of CREB protein 3)TORC3 Antibody (YA5086) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to TORC3.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB; IHC-P; IHC-F; ICC/IF; IF-Tissue
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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|---|---|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:100-200 | 1:50-200 | 1:50-200 |
Product Details
TORC3 Antibody (YA5086) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to TORC3.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 75 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 67 kDa
A synthesized peptide derived from human CRTC3. The exact sequence is proprietary to MCE.
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
TORC3 transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR)
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Subcellular Localization
Nucleus; Cytoplasm
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Expression
Tissue_specificity:It is primarily expressed in B cells and T cells. The highest expression level is found in lung tissue. It is also expressed in the brain, colon, heart, kidneys, ovaries, and prostate. Expression is weaker in the liver, pancreas, muscles, small intestine, spleen, and stomach. -
Isoforms & Post-Translational Modification
Q6UUV7 has 2 isomers: Q6UUV7-1: 66959 Da (predicted); Q6UUV7-3: 66830 Da (predicted).
Phosphorylation/dephosphorylation states of Ser-273 are required for regulating transduction of CREB activity (By similarity). CRTCs/TORCs are inactive when phosphorylated, and active when dephosphorylated at this site (By similarity). May be phosphorylated at Ser-391 by MAPK3/ERK1 and/or MAPK1/ERK2 or by some cyclin-dependent kinases such as CDK1,CDK2 or CDK5 (PubMed:30611118). Following adenylyl cyclase activation, dephosphorylated at Ser-162 and Ser-273 resulting in its dissociation from 14-3-3 proteins probably promoting CRTC3 translocation into the nucleus (PubMed:30611118) -
Subunit
Binding, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1 enhances recruitment of TAF4 to the promoter (PubMed:14506290). 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction (PubMed:14506290). Interacts (when phosphorylated at Ser-162 and Se-273) with 14-3-3 proteins (PubMed:30611118). Interacts with YWHAE (PubMed:30611118). Interacts (when phosphorylated at Ser-391) with phosphatase PP2A catalytic subunit PPP2CA and regulatory subunits PPP2R1A and PPP2R2A (PubMed:30611118). Interacts, via the N-terminal with the ankyrin repeats of BCL3, to form a complex with CREB1 on CRE and TxRE responsive elements and represses HTLV-1 LTR-mediated transcription (PubMed:17644518)
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SwissProt ID
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Synonyms
CRTC3; TORC3; CREB-regulated transcription coactivator 3; Transducer of regulated cAMP response element-binding protein 3; TORC-3; Transducer of CREB protein 3
Documentation
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