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Caspase-7 Antibody (YA4099)

(Synonyms: MCH3; CMH-1; LICE2; CASP7; ICE-LAP3)

Caspase-7 Antibody (YA4099) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Caspase-7.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Caspase-7 Antibody (YA4099)
  • Experimental Validation Results for Caspase-7 Antibody (YA4099)
  • Caspase-7 Antibody (YA4099) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

Caspase-7 Antibody (YA4099) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Caspase-7.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 34 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 34 kDa
Immunogen

Purified recombinant fragment of human CASP-7 (AA: 29-198) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Caspase-7 Antibody (YA4099)
    Western blot analysis of extracts from C1C12(lane 1(40μg) ),Raw264.7(lane 2(40μg) ),PC-12(lane 3(40μg) ),MCF-7(lane 4(40μg) ),MOLT4(lane 5(40μg) ),HEK293(lane 6(40μg) )and Jurkat(lane 7(40μg) ) using CASP-7 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Caspase-7 Antibody (YA4099)
    Flow cytometric analysis of 1X10^6 HepG2 cells labeling CASP-7 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Caspase 7 is an executioner cysteine protease that cleaves after aspartate residues and participates in programmed cell death and inflammation[1][2]. Mechanistically, death receptor and DNA-damage pathways activate caspase 7 through caspase 8 and caspase 9, placing it within apoptosis signaling cascades[1]. In Fas-stimulated Jurkat cells, caspase 8 activates caspase 3 and caspase 7, supporting a branched protease cascade during apoptotic execution[3]. In inflammatory models, caspase 1 inflammasomes activate caspase 7, and caspase 7 can mediate caspase 1-induced apoptosis upstream of caspase 3[1][4]. Disease and model evidence links caspase 7 to endotoxemia resistance in deficient mice, Salmonella-infected macrophages, neuropathic rat spinal cord apoptosis, and CHO-cell viability studies[1][4][5][6]. Compared with caspase 3, caspase 7 shares substrate recognition and some endogenous substrates, but specific substrates, caspase 1-dependent activation, and endotoxemia phenotypes distinguish the isoforms[1]. For experimental applications, isatin sulfonamides inhibit caspases 3 and 7 and serve as PET or SPECT apoptosis-imaging recognition units after radiolabeling[7].

  • Subcellular Localization

    Cytoplasm, cytosol; Nucleus; Secreted, extracellular space

  • Expression


    Tissue_specificity:It is highly expressed in the lungs, skeletal muscle, liver, kidneys, spleen, and heart, and moderately expressed in the testes. It is not expressed in the brain.

  • Isoforms & Post-Translational Modification

    P55210 has 4 isomers: P55210-1: 34277 Da (predicted); P55210-2: 28030 Da (predicted); P55210-3: 37815 Da (predicted); P55210-4: 31614 Da (predicted).
    Cleavage by different proteases, such as granzyme B (GZMB), caspase-1 (CASP1), caspase-8 (CASP8), caspase-9 (CASP9) or caspase-10 (CASP10) generate the two active subunits (PubMed:12824163, PubMed:16352606, PubMed:16916640, PubMed:35338844, PubMed:35446120, PubMed:9852092). Its involvement in different programmed cell death processes is probably specified by the protease that activates CASP7 (PubMed:16916640, PubMed:9852092). Cleaved and activated by initiator caspases (CASP8, CASP9 and/or CASP10), leading to execution phase of apoptosis (PubMed:16352606, PubMed:16916640, PubMed:21555521, PubMed:27889207). Cleavage and maturation by GZMB regulates granzyme-mediated programmed cell death (By similarity). Cleaved and activated by CASP1 in response to bacterial infection (By similarity). Propeptide domains can also be cleaved efficiently by CASP3 (PubMed:8755496). Active heterodimers between the small subunit of caspase-7 and the large subunit of CASP3, and vice versa, also occur (PubMed:8755496). Also cleaved at the N-terminus at alternative sites by CAPN1, leading to its activation (PubMed:19617626);Phosphorylation at Ser-30 and Ser-239 by PAK2 inhibits its activity (PubMed:21555521, PubMed:27889207). Phosphorylation at Ser-30 prevents cleavage and activation by initiator caspase CASP9, while phosphorylation at Ser-239 prevents thiol protease activity by preventing substrate-binding (PubMed:21555521, PubMed:27889207);(Microbial infection) ADP-riboxanation by C.violaceum CopC blocks CASP7 processing, preventing CASP7 activation and ability to recognize and cleave substrates;Ubiquitinated by BIRC6; this activity is inhibited by DIABLO/SMAC

  • Subunit

    Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 11 kDa (p11) subunit (PubMed:11701129, PubMed:11752425, PubMed:16916640, PubMed:20566630). Interacts with XIAP (via its second BIR domain); inhibiting CASP7 activity (PubMed:11257230, PubMed:11257231, PubMed:16916640). Interacts with BIRC6/bruce (PubMed:15200957). Interacts with ATXN3 (short isoform 1) (PubMed:30455355). Interacts with HSPA5 (PubMed:26045166)

  • SwissProt ID

    P55210

  • Gene ID
    840 [NCBI]
  • Synonyms

    MCH3; CMH-1; LICE2; CASP7; ICE-LAP3

[1]. Lamkanfi M, et al. Caspase-7: a protease involved in apoptosis and inflammation. Int J Biochem Cell Biol. 2010 Jan;42(1):21-4. [Content Brief]

[2]. Denault JB, et al. Caspases. Curr Protoc Protein Sci. 2002 Feb;Chapter 21:21.8.1-21.8.16. [Content Brief]

[3]. Hirata H, et al. Caspases are activated in a branched protease cascade and control distinct downstream processes in Fas-induced apoptosis. J Exp Med. 1998 Feb 16;187(4):587-600. [Content Brief]

[4]. Mahib MR, et al. Caspase-7 mediates caspase-1-induced apoptosis independently of Bid. Microbiol Immunol. 2020 Feb;64(2):143-152. [Content Brief]

[5]. Siniscalco D, et al. Involvement of subtype 1 metabotropic glutamate receptors in apoptosis and caspase-7 over-expression in spinal cord of neuropathic rats. Pharmacol Res. 2008 Mar;57(3):223-33. [Content Brief]

[6]. Safari F, et al. Caspase-7 deficiency in Chinese hamster ovary cells reduces cell proliferation and viability. Biol Res. 2020 Nov 13;53(1):52. [Content Brief]

[7]. Limpachayaporn P, et al. Isatin sulfonamides: potent caspases-3 and -7 inhibitors, and promising PET and SPECT radiotracers for apoptosis imaging. Future Med Chem. 2015;7(9):1173-96. [Content Brief]

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Caspase-7 Antibody (YA4099) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C1C12(lane 1(40μg) ),Raw264.7(lane 2(40μg) ),PC-12(lane 3(40μg) ),MCF-7(lane 4(40μg) ),MOLT4(lane 5(40μg) ),HEK293(lane 6(40μg) )and Jurkat(lane 7(40μg) ) using CASP-7 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HepG2 cells labeling CASP-7 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Caspase-7 Antibody (YA4099) Product Image
    1/16
  • Experimental Validation Results for Caspase-7 Antibody (YA4099)
  • Experimental Validation Results for Caspase-7 Antibody (YA4099)
  • Caspase-7 Antibody (YA4099) Product Image
  • Experimental Validation Results for Caspase-7 Antibody (YA4099)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C1C12(lane 1(40μg) ),Raw264.7(lane 2(40μg) ),PC-12(lane 3(40μg) ),MCF-7(lane 4(40μg) ),MOLT4(lane 5(40μg) ),HEK293(lane 6(40μg) )and Jurkat(lane 7(40μg) ) using CASP-7 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Caspase-7 Antibody (YA4099)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HepG2 cells labeling CASP-7 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Caspase-7 Antibody (YA4099)
  • Experimental Validation Results for Caspase-7 Antibody (YA4099)
  • Caspase-7 Antibody (YA4099) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C1C12(lane 1(40μg) ),Raw264.7(lane 2(40μg) ),PC-12(lane 3(40μg) ),MCF-7(lane 4(40μg) ),MOLT4(lane 5(40μg) ),HEK293(lane 6(40μg) )and Jurkat(lane 7(40μg) ) using CASP-7 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Caspase-7 Antibody (YA4099) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 HepG2 cells labeling CASP-7 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Caspase-7 Antibody (YA4099) Product Image WB
  • Caspase-7 Antibody (YA4099) Product Image FC