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CHRM4 Antibody

(Synonyms: Muscarinic acetylcholine receptor M4, CHRM4)

CHRM4 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to CHRM4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CHRM4 Antibody
  • CHRM4 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-2000 1:100-500

Product Details

Description

CHRM4 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to CHRM4.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 53 kDa
Immunogen

Synthetic peptide corresponding to the center region of human CHRM4.

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CHRM4 Antibody
    Western blot analysis of extracts from MCF-7 (lane 2, 20ug), Hela (lane 3, 20ug) and A549 (lane 4, 20ug) using CHRM4 Antibody (HY-P811189). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    CHRM4 is a The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase

  • Subcellular Localization

    Cell membrane; Postsynaptic cell membrane

  • Isoforms & Post-Translational Modification

    CHRM4 has an amino acid length of 479, molecular weight is 53049 Da.

  • SwissProt ID

    P08173

  • Gene ID
    1132 [NCBI]
  • Synonyms

    Muscarinic acetylcholine receptor M4, CHRM4

CHRM4 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane 2, 20ug), Hela (lane 3, 20ug) and A549 (lane 4, 20ug) using CHRM4 Antibody (HY-P811189). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • CHRM4 Antibody Product Image
    1/16
  • Experimental Validation Results for CHRM4 Antibody
  • CHRM4 Antibody Product Image
  • Experimental Validation Results for CHRM4 Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane 2, 20ug), Hela (lane 3, 20ug) and A549 (lane 4, 20ug) using CHRM4 Antibody (HY-P811189). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CHRM4 Antibody
  • CHRM4 Antibody Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7 (lane 2, 20ug), Hela (lane 3, 20ug) and A549 (lane 4, 20ug) using CHRM4 Antibody (HY-P811189). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • CHRM4 Antibody Product Image WB