PHLDA1 Antibody (YA1616)

Cat. No.: HY-P81871: Ficha de datos COA
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User Guide for Antibodies
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PHLDA1 Antibody (YA1616) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHLDA1.

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Tamaño	Stock	Cantidad
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10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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PHLDA1 Antibody (YA1616) Recomendaciones destacadas:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Descripciòn

Descripciòn

PHLDA1 Antibody (YA1616) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHLDA1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Peso molecular

Predicted band size: 45 kDa;

Observed band size: 45 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q8WV24

Gene ID

22822 [NCBI]

Immunogen

A synthetic peptide of human PHLDA1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Pureza	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from A375 (lane 1, 15 μg), HT-1080 (lane 2, 15 μg), and U-87MG (lane 3, 15 μg) using PHLDA1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Seems to be involved in regulation of apoptosis. May be involved in detachment-mediated programmed cell death. May mediate apoptosis during neuronal development. May be involved in regulation of anti-apoptotic effects of IGF1. May be involved in translational regulation

Subcellular Localization：Cytoplasm; Cytoplasmic vesicle; Nucleus, nucleolus

Expression：
Tissue_specificity:Widely expressed with highest levels in pancreas. Strongly expressed by benign melanocytic nevi, and progressively reduced expressed in primary and metastatic melanomas (at protein level)

Induction:Induced by homocysteine and other endoplasmic reticulum stress-inducing reagents. Induced by phorbol ester (TPA) /ionomycin, and stimulation of the T-cell receptor (TCR) complex in T-cells

Subunit：Interacts with RPL14, EIF3S7 and PABPC4

RRID

AB_3103969

Database

Entrez Gene: 22822 Human

SwissProt: Q8WV24 Human

OMIM: 605335 Human

Research Field

Cell Biology

Synonyms

PQ-rich protein; PQR protein; Proline- and histidine-rich protein; T-cell death-associated gene 51 protein

Documentación

Select Batch:

Ficha de datos (234 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

PHLDA1 Antibody (YA1616) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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PHLDA1 Antibody (YA1616)

PHLDA1 Antibody (YA1616) Recomendaciones destacadas:

Product Detail

Verification Image

Background

Descripciòn

PHLDA1 Antibody (YA1616) Related Classifications

Productos vistos recientemente:

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