AF10 Antibody (YA2017)(PBS only)

Cat. No.: HY-P82272A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

AF10 Antibody (YA2017) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to AF10.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

AF10 Antibody (YA2017) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to AF10.

Host

Rabbit

Molecular Weight

Predicted band size: 113 kDa;

Observed band size: 140 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P55197

Gene ID

8028 [NCBI]

Immunogen

Recombinant protein of human AF10 aa788-1050.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Probably involved in transcriptional regulation. In vitro or as fusion protein with KMT2A/MLL1 has transactivation activity. Binds to cruciform DNA. In cells, binding to unmodified histone H3 regulates DOT1L functions including histone H3 'Lys-79' dimethylation (H3K79me2) and gene activation (PubMed:26439302)

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:Expressed abundantly in testis

Isoforms & Post-Translational Modification：P55197 has 4 isomers: P55197-4: 113320 Da (predicted); P55197-1: 109026 Da (predicted); P55197-2: 13851 Da (predicted); P55197-3: 20047 Da (predicted).

Subunit：Self-associates. Interacts with FSTL3 isoform 2; the interaction enhances MLLT10 in vitro transcriptional activity and self-association. Interacts with YEATS4.

Research Field

Epigenetics and Nuclear Signaling

Synonyms

MLLT10; AF10; Protein AF-10; ALL1-fused gene from chromosome 10 protein

Documentation

User Guide for Antibodies (1077 KB)