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PGD Antibody (YA1727)

(Synonyms: 6-phosphogluconate dehydrogenase; decarboxylating)
Customer Review

Based on 1 Customer Validation

PGD Antibody (YA1727) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PGD.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL ICC WB
  • Experimental Validation Results for PGD Antibody (YA1727)
  • Experimental Validation Results for PGD Antibody (YA1727)
  • Experimental Validation Results for PGD Antibody (YA1727)
  • PGD Antibody (YA1727) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

PGD Antibody (YA1727) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PGD.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 49 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 53 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human PGD

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PGD Antibody (YA1727)
    Immunocytochemistry analysis of Hela cells labeling PGD with PGD Antibody (HY-P81982) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PGD Antibody (HY-P81982) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PGD Antibody (YA1727)
    Immunocytochemistry analysis of U2OS cells labeling PGD with PGD Antibody (HY-P81982) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PGD Antibody (HY-P81982) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PGD Antibody (YA1727)
    Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), HeLa (lane 2, 15 μg), Mouse brain (lane 3, 15 μg), Rat brain (lane 4, 15 μg), Raji (lane 5, 15 μg), and MOLT-4 (lane 6, 15 μg) using PGD Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:2000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    PGD catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH

  • Subcellular Localization

    Cytoplasm

  • Isoforms & Post-Translational Modification

    P52209 has 2 isomers: P52209-1: 53140 Da (predicted); P52209-2: 51872 Da (predicted).

  • Subunit

    Homodimer

  • SwissProt ID

    P52209

  • Gene ID
    5226 [NCBI]
  • Synonyms

    6-phosphogluconate dehydrogenase; decarboxylating

  • Research Field

    Signal Transduction

PGD Antibody (YA1727) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PGD with PGD Antibody (HY-P81982) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PGD Antibody (HY-P81982) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of U2OS cells labeling PGD with PGD Antibody (HY-P81982) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PGD Antibody (HY-P81982) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), HeLa (lane 2, 15 μg), Mouse brain (lane 3, 15 μg), Rat brain (lane 4, 15 μg), Raji (lane 5, 15 μg), and MOLT-4 (lane 6, 15 μg) using PGD Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:2000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • PGD Antibody (YA1727) Product Image
    1/16
  • Experimental Validation Results for PGD Antibody (YA1727)
  • Experimental Validation Results for PGD Antibody (YA1727)
  • Experimental Validation Results for PGD Antibody (YA1727)
  • PGD Antibody (YA1727) Product Image
  • Experimental Validation Results for PGD Antibody (YA1727)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PGD with PGD Antibody (HY-P81982) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PGD Antibody (HY-P81982) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PGD Antibody (YA1727)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of U2OS cells labeling PGD with PGD Antibody (HY-P81982) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PGD Antibody (HY-P81982) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PGD Antibody (YA1727)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), HeLa (lane 2, 15 μg), Mouse brain (lane 3, 15 μg), Rat brain (lane 4, 15 μg), Raji (lane 5, 15 μg), and MOLT-4 (lane 6, 15 μg) using PGD Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:2000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for PGD Antibody (YA1727)
  • Experimental Validation Results for PGD Antibody (YA1727)
  • Experimental Validation Results for PGD Antibody (YA1727)
  • PGD Antibody (YA1727) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling PGD with PGD Antibody (HY-P81982) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PGD Antibody (HY-P81982) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PGD Antibody (YA1727) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of U2OS cells labeling PGD with PGD Antibody (HY-P81982) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PGD Antibody (HY-P81982) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PGD Antibody (YA1727) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), HeLa (lane 2, 15 μg), Mouse brain (lane 3, 15 μg), Rat brain (lane 4, 15 μg), Raji (lane 5, 15 μg), and MOLT-4 (lane 6, 15 μg) using PGD Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:2000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • PGD Antibody (YA1727) Product Image ICC
  • PGD Antibody (YA1727) Product Image ICC
  • PGD Antibody (YA1727) Product Image WB