Synapsin I Antibody (YA3835)(PBS only)

Cat. No.: HY-P84138A: Data Sheet User Guide for Antibodies
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Synapsin I Antibody (YA3835) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Synapsin I.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Synapsin I Antibody (YA3835) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Synapsin I.

Host

Mouse

Molecular Weight

Predicted band size: 74 kDa;

Observed band size: 74 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Monkey, Rat

SwissProt ID

P17600

Gene ID

6853 [NCBI]

Immunogen

Purified recombinant fragment of human SYN1 (AA: 362-511) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Neuronal phosphoprotein that coats synaptic vesicles, and binds to the cytoskeleton. Acts as a regulator of synaptic vesicles trafficking, involved in the control of neurotransmitter release at the pre-synaptic terminal (PubMed:21441247, PubMed:23406870). Also involved in the regulation of axon outgrowth and synaptogenesis (By similarity). The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level (By similarity)

Subcellular Localization：Synapse; Golgi apparatus; Presynapse; Cytoplasmic vesicle, secretory vesicle, synaptic vesicle

Isoforms & Post-Translational Modification：P17600 has 2 isomers: P17600-1: 74111 Da (predicted); P17600-2: 70033 Da (predicted).
Substrate of different protein kinases. Phosphorylated by CaMK2 and MAPK1 (PubMed:21441247). Phosphorylation, including phosphorylation at Ser-9, promotes synapsin-1 dissociation from synaptic vesicles, regulates its rate of dispersion, and controls the kinetics of vesicle pool turnover and neurotransmitter release (By similarity) (PubMed:21441247)

Subunit：Homodimer (By similarity). Can form oligomers with SYN2 (PubMed:23406870). Interacts with CAPON. Forms a ternary complex with NOS1 (By similarity). Isoform Ib interacts with PRNP (By similarity)

Synonyms

SYNI; SYN1a; SYN1b

Documentation

User Guide for Antibodies (1077 KB)