NMDAR1 Antibody (YA7197)

Cat. No.: HY-P87513: Data Sheet User Guide for Antibodies
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NMDAR1 Antibody (YA7197) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NMDAR1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

NMDAR1 Antibody (YA7197) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NMDAR1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 103 kDa;

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q05586

Immunogen

A synthesized peptide derived from human GluN1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:100-500

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M tris buffered saline (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed:21376300, PubMed:26875626, PubMed:26919761, PubMed:28126851, PubMed:28228639, PubMed:36959261, PubMed:7679115, PubMed:7681588, PubMed:7685113). NMDARs participate in synaptic plasticity for learning and memory formation by contributing to the long-term potentiation (LTP) (PubMed:26875626). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:21376300, PubMed:26875626, PubMed:26919761, PubMed:27164704, PubMed:28095420, PubMed:28105280, PubMed:28126851, PubMed:28228639, PubMed:36959261, PubMed:38538865, PubMed:7679115, PubMed:7681588, PubMed:7685113). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium (By similarity). Each GluN2 or GluN3 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed:26875626, PubMed:26919761, PubMed:36309015, PubMed:38598639)

Subcellular Localization：Cell membrane,Postsynaptic cell membrane,Postsynaptic density membrane,Synaptic cell membrane

Isoforms & Post-Translational Modification：Q05586 has 7 isomers: Q05586-1: 105373 Da (predicted); Q05586-2: 99315 Da (predicted); Q05586-3: 101209 Da (predicted); Q05586-4: 103479 Da (predicted); Q05586-5: 107909 Da (predicted); Q05586-6: 106015 Da (predicted); Q05586-7: 101851 Da (predicted).
NMDA is probably regulated by C-terminal phosphorylation of an isoform of GRIN1 by PKC (PubMed:8316301)

Subunit：Heterotetramer; the NMDAR subunits are modular and harbor tiered domains that function in concert to regulate opening and closing of the cation-selective ion channel pore (PubMed:36309015, PubMed:38598639)

Documentation

User Guide for Antibodies (1077 KB)