Hexokinase II Antibody (YA4082)(PBS only)

Cat. No.: HY-P84385A: Data Sheet User Guide for Antibodies
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Hexokinase II Antibody (YA4082) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Hexokinase II.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Hexokinase II Antibody (YA4082) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Hexokinase II.

Host

Mouse

Molecular Weight

Predicted band size: 102 kDa;

Observed band size: 102 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Rabbit

SwissProt ID

P52789

Gene ID

3099 [NCBI]

Immunogen

Purified recombinant fragment of human HK2 aa 264-505.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:500
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively) (PubMed:23185017, PubMed:26985301, PubMed:29298880). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:29298880). Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis (PubMed:18350175)

Subcellular Localization：Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasm, cytosol

Expression：
Tissue_specificity:The main hexokinase isoenzyme expressed in insulin-sensitive tissues such as skeletal muscle

Subunit：Monomer (By similarity). Interacts with TIGAR; the interaction increases hexokinase activity in a hypoxia- and HIF1A-dependent manner (PubMed:23185017)

Synonyms

HKII; HXK2; DKFZp686M1669; HK2

Documentation

User Guide for Antibodies (1077 KB)