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hnRNP A1 Antibody (YA2484)

(Synonyms: HNRNPA 1; HNRNPA1; HNRPA1)
Customer Review

Based on 1 Customer Validation

hnRNP A1 Antibody (YA2484) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to hnRNP A1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P FC
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • hnRNP A1 Antibody (YA2484) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50-1:100

Product Details

Description

hnRNP A1 Antibody (YA2484) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to hnRNP A1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 33 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 39 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human hnRNP A1 aa301-350.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104837

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    Western blot analysis was performed on extracts from MCF-7 (lane 1, 15 μg), HepG2 (lane 2, 15 μg), 293 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and 3T3 (lane 5, 15 μg) using hnRNP A1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    Immunohistochemical analysis of paraffin-embedded human epididymis tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    Immunohistochemical analysis of paraffin-embedded human bronchus tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    Immunohistochemical analysis of paraffin-embedded human oral mucosa tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    Immunohistochemical analysis of paraffin-embedded human testis tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    Immunohistochemical analysis of paraffin-embedded mouse pancreas using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    Immunohistochemical analysis of paraffin-embedded mouse epididymis tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    Flow cytometric analysis of 1X106 Jurkat cells labeling hnRNP A1 Antibody (HY-P82739, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    hnRNP A1 is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and modulation of splice site selection. Plays a role in the splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform. Binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1. May bind to specific miRNA hairpins; (Microbial infection) May play a role in HCV RNA replication; (Microbial infection) Cleavage by Enterovirus 71 protease 3C results in increased translation of apoptosis protease activating factor APAF1, leading to apoptosis[1][2][3][4].

  • Subcellular Localization

    Nucleus; Cytoplasm; Cytoplasm; Nucleus

  • Isoforms & Post-Translational Modification

    P09651 has 3 isomers: P09651-1: 38747 Da (predicted); P09651-2: 34196 Da (predicted); P09651-3: 29386 Da (predicted).
    Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine;Sumoylated

  • Subunit

    Identified in the spliceosome C complex (PubMed:11991638). Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs (PubMed:17289661). Interacts with SEPT6 (PubMed:17229681). Interacts with C9orf72 (PubMed:24549040). Interacts with KHDRBS1 (PubMed:17371836). Interacts with UBQLN2 (PubMed:25616961). Interacts with PPIA/CYPA (PubMed:25678563). Interacts (via the RGG-box) with the HOXB-AS3 peptide; the interaction inhibits binding of HNRNPA1 to the intronic sequences flanking exon 9 of the PKM gene, preventing inclusion of exon 9 and promoting inclusion of exon 10 which suppresses formation of the PKM M2 isoform and promotes production of the M1 isoform (PubMed:28985503)

  • SwissProt ID

    P09651

  • Gene ID
    3178 [NCBI]
  • Synonyms

    HNRNPA 1; HNRNPA1; HNRPA1

  • Research Field

    Epigenetics and Nuclear Signaling

References

[1]. Paronetto MP, et al. The RNA-binding protein Sam68 modulates the alternative splicing of Bcl-x. J Cell Biol. 2007 Mar 26;176(7):929-39. [Content Brief]

[2]. David CJ, et al. HnRNP proteins controlled by c-Myc deregulate pyruvate kinase mRNA splicing in cancer. Nature. 2010 Jan 21;463(7279):364-8. [Content Brief]

[3]. Li ML, et al. EV71 3C protease induces apoptosis by cleavage of hnRNP A1 to promote apaf-1 translation. PLoS One. 2019;14(9):e0221048. [Content Brief]

[4]. Treiber T, et al. A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis. Mol Cell. 2017 Apr 20;66(2):270-284.e13. [Content Brief]

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hnRNP A1 Antibody (YA2484) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from MCF-7 (lane 1, 15 μg), HepG2 (lane 2, 15 μg), 293 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and 3T3 (lane 5, 15 μg) using hnRNP A1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human epididymis tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bronchus tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human small intestine tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human oral mucosa tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse pancreas using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse lung tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse epididymis tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Jurkat cells labeling hnRNP A1 Antibody (HY-P82739, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • hnRNP A1 Antibody (YA2484) Product Image
    1/16
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • hnRNP A1 Antibody (YA2484) Product Image
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from MCF-7 (lane 1, 15 μg), HepG2 (lane 2, 15 μg), 293 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and 3T3 (lane 5, 15 μg) using hnRNP A1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human epididymis tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bronchus tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human small intestine tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human oral mucosa tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse pancreas using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse lung tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse epididymis tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Jurkat cells labeling hnRNP A1 Antibody (HY-P82739, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • Experimental Validation Results for hnRNP A1 Antibody (YA2484)
  • hnRNP A1 Antibody (YA2484) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from MCF-7 (lane 1, 15 μg), HepG2 (lane 2, 15 μg), 293 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and 3T3 (lane 5, 15 μg) using hnRNP A1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • hnRNP A1 Antibody (YA2484) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human epididymis tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • hnRNP A1 Antibody (YA2484) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • hnRNP A1 Antibody (YA2484) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human bronchus tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • hnRNP A1 Antibody (YA2484) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • hnRNP A1 Antibody (YA2484) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human oral mucosa tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • hnRNP A1 Antibody (YA2484) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human testis tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • hnRNP A1 Antibody (YA2484) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • hnRNP A1 Antibody (YA2484) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse pancreas using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • hnRNP A1 Antibody (YA2484) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • hnRNP A1 Antibody (YA2484) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse epididymis tissue using hnRNP A1 Antibody (HY-P82739, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • hnRNP A1 Antibody (YA2484) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 Jurkat cells labeling hnRNP A1 Antibody (HY-P82739, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • hnRNP A1 Antibody (YA2484) Product Image WB
  • hnRNP A1 Antibody (YA2484) Product Image IHC-P
  • hnRNP A1 Antibody (YA2484) Product Image IHC-P
  • hnRNP A1 Antibody (YA2484) Product Image IHC-P
  • hnRNP A1 Antibody (YA2484) Product Image IHC-P
  • hnRNP A1 Antibody (YA2484) Product Image IHC-P
  • hnRNP A1 Antibody (YA2484) Product Image IHC-P
  • hnRNP A1 Antibody (YA2484) Product Image IHC-P
  • hnRNP A1 Antibody (YA2484) Product Image IHC-P
  • hnRNP A1 Antibody (YA2484) Product Image IHC-P
  • hnRNP A1 Antibody (YA2484) Product Image IHC-P
  • hnRNP A1 Antibody (YA2484) Product Image FC