CYP7B1 Antibody (YA7801)(PBS only)
(Synonyms: CBAS3; CP7B; SPG5A)CYP7B1 Antibody (YA7801) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CYP7B1.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, IHC-P
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:50-5000 |
Product Details
CYP7B1 Antibody (YA7801) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CYP7B1.
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Host Mouse
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 58.1 kDa
Human recombinant protein fragment corresponding to amino acids 263-506 of human CYP7B1 produced in E.coli.
Affinity purified
Non-conjugated
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CYP7B1 is an A cytochrome P450 monooxygenase involved in the metabolism of endogenous oxysterols and steroid hormones, including neurosteroids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon hydrogen bonds of steroids with a preference for 7-alpha position. Usually metabolizes steroids carrying a hydroxy group at position 3, functioning as a 3-hydroxy steroid 7-alpha hydroxylase. Hydroxylates oxysterols, including 25-hydroxycholesterol and (25R)-cholest-5-ene-3beta,26-diol toward 7-alpha hydroxy derivatives, which may be transported to the liver and converted to bile acids. Via its product 7-alpha,25-dihydroxycholesterol, a ligand for the chemotactic G protein-coupled receptor GPR183/EBI2, regulates B cell migration in germinal centers of lymphoid organs, thus guiding efficient maturation of plasma B cells and overall antigen-specific humoral immune response. 7-alpha hydroxylates neurosteroids, including 3beta-hydroxyandrost-5-en-17-one (dehydroepiandrosterone) and pregnenolone, both involved in hippocampus-associated memory and learning. Metabolizes androstanoids toward 6- or 7-alpha hydroxy derivatives[1][2][3].
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Subcellular Localization
Endoplasmic reticulum membrane,Microsome membrane
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Expression
Tissue_Specificity: Widely expressed. Expressed in brain, testis, ovary, prostate, liver, colon, kidney, small intestine, thymus and spleen -
Isoforms & Post-Translational Modification
O75881: 506 amino acids, molecular weight 58256 Da.
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SwissProt ID
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Synonyms
CBAS3; CP7B; SPG5A
Documentation
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