Collagen II Antibody(YA3550)
(Synonyms: Collagen II alpha 1; COL2A1; COL2A1 protein; collagen, type II, alpha 1; collagen alpha-1(II); type II collagen; alpha-1 type II collagen; alpha1 type II collagen; Col2a1; AOM; Cartilage collagen; Chondrocalcin; COL11A3; Collagen alpha 1(II) chain precursor; Collagen II alpha 1 polypeptide; Collagen type II alpha 1 (primary osteoarthritis spondyloepiphyseal dysplasia congenital); MGC131516; SEDC; Collagen alpha-1(II) chain; Alpha-1 type II collagen; CO2A1_HUMAN.; CollagenII; )Based on 1 publication(s) in Google Scholar
Collagen II Antibody(YA3550) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Collagen II.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IF-Tissue
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Publications Citing Use of MedChemExpress (MCE) Collagen II Antibody(YA3550)
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Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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|---|---|---|---|
| Dilution Ratio | 1:1000 | 1:1000 | 1:200 |
Product Details
Collagen II Antibody(YA3550) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Collagen II.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 142 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 117 KDa
Synthetic peptide within human Collagen II aa 501-600.
Affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Publications (1)
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Journal Impact Factor
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Most Recent
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ACS Biomater Sci Eng
Physiological Mechanical Loading Is Crucial for Chondrogenesis and Cartilage Formation by Human Synovium-Derived Stem Cells Stimulated by Kartogenin. [Abstract]2026 Mar 9;12(3):1647-1662. PMID: 41717818
Verification Images
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Western blot analysis of extracts from RAW264.7(lane 2(20ug) , THP-1(lane 3(20ug) and NIH/3T3(lane 4(20ug) , HepG2(lane 5(20ug) using Collagen II Antibody (HY-P81047A) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Background
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Function
Collagen II is the major collagen synthesized by chondrocytes and a core structural component of articular cartilage, vitreous humour, inner ear, and nucleus pulposus[1]. Mechanistically, COL2A1 suppresses articular chondrocyte hypertrophy and osteoarthritis progression by promoting integrin β1-SMAD1 interaction and regulating BMP-SMAD1 signaling[2]. In disease models, COL2A1 loss accelerates chondrocyte hypertrophy, while degenerative human articular cartilage shows reduced COL2A1 with increased hypertrophic markers and BMP-SMAD1 activity[2]. Type II collagen degradation can also expose a cryptic peptide sequence that induces collagenase activity, MMP-13, and chondrocyte hypertrophy in articular cartilage explants[3]. Compared with type X collagen, which marks hypertrophic chondrocytes in osteoarthritis, collagen II better reflects the stable hyaline cartilage phenotype[4]. Compared with collagen I devices, collagen II biomaterials more directly match cartilage extracellular matrix composition and support cartilage engineering research[5]. For experimental applications, collagen II scaffolds help maintain or rejuvenate hyaline chondrocyte phenotype through Wnt-related mechanisms, supporting use in cartilage tissue engineering models[6].
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Subcellular Localization
Secreted, extracellular space, extracellular matrix
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Expression
Tissue_specificity:Isomer 2 is highly expressed in juvenile chondrocytes and lowly expressed in fetal chondrocytes.
Positive sample: Human cartilage tissue, mouse cartilage tissue, rat cartilage tissue. -
Isoforms & Post-Translational Modification
P02458 has 3 isomers: P02458-2: 141785 Da (predicted); P02458-1: 134389 Da (predicted); P02458-3: 29781 Da (predicted).
The N-telopeptide is covalently linked to the helical COL2 region of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chain. The C-telopeptide is covalently linked to an another site in the helical region of alpha 3(IX) COL2;Contains mostly 4-hydroxyproline. Prolines at the third position of the tripeptide repeating unit (G-X-P) are 4-hydroxylated in some or all of the chains;Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline;Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains;O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide -
Subunit
Homotrimers of alpha 1(II) chains
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SwissProt ID
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Synonyms
Collagen II alpha 1; COL2A1; COL2A1 protein; collagen, type II, alpha 1; collagen alpha-1(II); type II collagen; alpha-1 type II collagen; alpha1 type II collagen; Col2a1; AOM; Cartilage collagen; Chondrocalcin; COL11A3; Collagen alpha 1(II) chain precursor; Collagen II alpha 1 polypeptide; Collagen type II alpha 1 (primary osteoarthritis spondyloepiphyseal dysplasia congenital); MGC131516; SEDC; Collagen alpha-1(II) chain; Alpha-1 type II collagen; CO2A1_HUMAN.; CollagenII;
Documentation
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Data Sheet (261 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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User Guide for Antibodies (1077 KB)
References
[1]. Norhalifah HK, et al. The genetic history of Peninsular Malaysia. Gene. 2016 Jul 15;586(1):129-35. [Content Brief]
[2]. Lian C, et al. Collagen type II suppresses articular chondrocyte hypertrophy and osteoarthritis progression by promoting integrin β1-SMAD1 interaction. Bone Res. 2019 Mar 6;7:8. [Content Brief]
[3]. van der Kraan PM, et al. Chondrocyte hypertrophy and osteoarthritis: role in initiation and progression of cartilage degeneration? Osteoarthritis Cartilage. 2012 Mar;20(3):223-32. [Content Brief]
[4]. Wu Z, et al. Collagen type II: From biosynthesis to advanced biomaterials for cartilage engineering. Biomater Biosyst. 2021 Nov 22;4:100030. [Content Brief]
[5]. Zhou H, et al. Rejuvenating Hyaline Cartilaginous Phenotype of Dedifferentiated Chondrocytes in Collagen II Scaffolds: A Mechanism Study Using Chondrocyte Membrane Nanoaggregates as Antagonists. ACS Nano. 2024 Jan 23;18(3):2077-2090. [Content Brief]
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