BNIP1 Antibody (YA3329)
(Synonyms: BCL2 adenovirus E1B 19kD interacting protein 1; BNIP1; Nip1; sec20; SEC20L; Transformation-related gene 8 protein; TRG8; Vesicle transport protein SEC20)BNIP1 Antibody (YA3329) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BNIP1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 |
Product Details
BNIP1 Antibody (YA3329) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BNIP1.
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Host Rabbit
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Clonality Recombinant, Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 26 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 26 kDa
Entrez Gene: 662 Human ; 224630 Mouse ; 140932 Rat
SwissProt: Q12981 Human ; Q6QD59 Mouse ; Q8VHI8 Rat
OMIM: 603291 Human
A synthesized peptide derived from human BNIP1 aa100-150/228.
Endogenous
Affinity Chromatography
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
BNIP1 is an As part of a SNARE complex may be involved in endoplasmic reticulum membranes fusion and be required for the maintenance of endoplasmic reticulum organization. Also plays a role in apoptosis. It is for instance required for endoplasmic reticulum stress-induced apoptosis. As a substrate of RNF185 interacting with SQSTM1, might also be involved in mitochondrial autophagy (Probable)[1][2][3].
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Subcellular Localization
Endoplasmic reticulum membrane; Single-pass type IV membrane protein; Mitochondrion membrane; Single-pass type IV membrane protein
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Expression
Tissue_specificity:Isoform 1 is highly expressed in heart, brain, liver skeletal muscle and pancreas. Isoform 3 is moderately expressed in placenta, lung and kidney. Isoform 4 is highly expressed in testis and small intestine -
Isoforms & Post-Translational Modification
Q12981 has 4 isomers: Q12981-4: 26132 Da (predicted); Q12981-2: 22352 Da (predicted); Q12981-1: 31113 Da (predicted); Q12981-3: 27333 Da (predicted).
Polyubiquitinated (PubMed:21931693, PubMed:23896122). 'Lys-63'-linked polyubiquitination by RNF185 increases the interaction with the autophagy receptor SQSTM1 (PubMed:21931693). Undergoes 'Lys-29'- and 'Lys-63'-linked polyubiquitination by RNF186 that may regulate BNIP1 localization to the mitochondrion (PubMed:23896122) -
Subunit
Component of a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L and SEC22B (PubMed:15272311). Interacts directly with STX18, RINT1/TIP20L and NAPA (PubMed:15272311). Interacts with ZW10 through RINT1 (PubMed:15272311). Interacts with BCL2 (PubMed:7954800). Interacts with RNF186 (PubMed:23896122). Interacts with RNF185 (PubMed:21931693). Interacts with SQSTM1; increased by 'Lys-63'-linked polyubiquitination of BNIP1 (PubMed:21931693)
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SwissProt ID
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Synonyms
BCL2 adenovirus E1B 19kD interacting protein 1; BNIP1; Nip1; sec20; SEC20L; Transformation-related gene 8 protein; TRG8; Vesicle transport protein SEC20
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Research Field
Cell Biology
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