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NeuN Antibody (YA3480)(PBS only)

(Synonyms: FOX3; NEUN; FOX-3; HRNBP3)

NeuN Antibody (YA3480) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NeuN.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC, ELISA

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for NeuN Antibody (YA3480)(PBS only)
  • NeuN Antibody (YA3480)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

NeuN Antibody (YA3480) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NeuN.

  • Host Mouse
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 46-55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 34 kDa
Immunogen

Purified recombinant fragment of human RBFOX3 (AA: 1-140) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NeuN Antibody (YA3480)(PBS only)
    Western blot analysis of extracts from C2C12(lane 2(20ug) , RAW264.7(lane 3(20ug) and U251(lane 4(20ug) , NIH/3T3(lane 5(20ug) using NeuN Antibody (HY-P83783) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    NeuN is a Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD)

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Isoforms & Post-Translational Modification

    A6NFN3 has 2 isomers: A6NFN3-1: 33873 Da (predicted); A6NFN3-2: 35123 Da (predicted).

  • SwissProt ID

    A6NFN3

  • Gene ID
    146713 [NCBI]
  • Synonyms

    FOX3; NEUN; FOX-3; HRNBP3

NeuN Antibody (YA3480)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C2C12(lane 2(20ug) , RAW264.7(lane 3(20ug) and U251(lane 4(20ug) , NIH/3T3(lane 5(20ug) using NeuN Antibody (HY-P83783) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • NeuN Antibody (YA3480)(PBS only) Product Image
    1/16
  • Experimental Validation Results for NeuN Antibody (YA3480)(PBS only)
  • NeuN Antibody (YA3480)(PBS only) Product Image
  • Experimental Validation Results for NeuN Antibody (YA3480)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C2C12(lane 2(20ug) , RAW264.7(lane 3(20ug) and U251(lane 4(20ug) , NIH/3T3(lane 5(20ug) using NeuN Antibody (HY-P83783) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NeuN Antibody (YA3480)(PBS only)
  • NeuN Antibody (YA3480)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C2C12(lane 2(20ug) , RAW264.7(lane 3(20ug) and U251(lane 4(20ug) , NIH/3T3(lane 5(20ug) using NeuN Antibody (HY-P83783) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • NeuN Antibody (YA3480)(PBS only) Product Image WB