Copy Link Print Information

MLD Antibody (YA3235)(PBS only)

(Synonyms: MLD; DEGS; DES1; Des-1; FADS7; MIG15; DEGS-1)

MLD Antibody (YA3235) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MLD.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MLD Antibody (YA3235)(PBS only)
  • MLD Antibody (YA3235)(PBS only) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

MLD Antibody (YA3235) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MLD.

  • Host Rabbit
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 38 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 38 kDa
Immunogen

A synthetic peptide of human MLD

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MLD Antibody (YA3235)(PBS only)
    Western blot analysis of extracts from HEK293T(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using MLD Antibody (HY-P83490) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    MLD is a Has sphingolipid-delta-4-desaturase activity. Converts D-erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine). Catalyzes the equilibrium isomerization of retinols[1][2][3].

  • Subcellular Localization

    Mitochondrion membrane; Endoplasmic reticulum membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Ubiquitous

  • Subunit

    Interacts with RLBP1; the interaction increases synthesis of chromophore-precursors by DEGS1

  • SwissProt ID

    O15121

  • Gene ID
    8560 [NCBI]
  • Synonyms

    MLD; DEGS; DES1; Des-1; FADS7; MIG15; DEGS-1

  • Research Field

    Cardiovascular

MLD Antibody (YA3235)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using MLD Antibody (HY-P83490) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • MLD Antibody (YA3235)(PBS only) Product Image
    1/16
  • Experimental Validation Results for MLD Antibody (YA3235)(PBS only)
  • MLD Antibody (YA3235)(PBS only) Product Image
  • Experimental Validation Results for MLD Antibody (YA3235)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using MLD Antibody (HY-P83490) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MLD Antibody (YA3235)(PBS only)
  • MLD Antibody (YA3235)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293T(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using MLD Antibody (HY-P83490) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • MLD Antibody (YA3235)(PBS only) Product Image WB