Caprin1 Antibody (YA9301)
(Synonyms: GPIAP1, GPIP137, M11S1, RNG105, CAPRIN1, Caprin-1, Cell cycle-associated protein 1, Cytoplasmic activation- and proliferation-associated protein 1, GPI-anchored membrane protein 1, GPI-anchored protein p137, Membrane component chromosome 11 surface marker 1, RNA granule protein 105, GPI-p137, p137GPI)
Based on 1 Customer Validation
Caprin1 Antibody (YA9301) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Caprin1.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, ICC/IF, IF-Tissue, IP, ELISA
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Reactivity :
human
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Formulation:
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IP
IP: Immunoprecipitation
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:50-500 | 1:50-500 | 1-2μg per 100-500μg Total protein | 1:50-3000 |
Product Details
Caprin1 Antibody (YA9301) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Caprin1.
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Host Mouse
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Species Reactivityhuman
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Calculated Molecular Weight Predicted band size: 78 kDa
OMIM: 601178
A synthesized peptide derived from human Caprin1.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
Caprin1 is a mRNA-binding protein that acts as a regulator of mRNAs transport, translation and/or stability, and which is involved in neurogenesis, synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Plays an essential role in cytoplasmic stress granule formation. Acts as an mRNA regulator by mediating formation of some phase-separated membraneless compartment: undergoes liquid-liquid phase separation upon binding to target mRNAs, leading to assemble mRNAs into cytoplasmic ribonucleoprotein granules that concentrate mRNAs with associated regulatory factors. Undergoes liquid-liquid phase separation following phosphorylation and interaction with FMR1, promoting formation of cytoplasmic ribonucleoprotein granules that concentrate mRNAs with factors that inhibit translation and mediate deadenylation of target mRNAs. In these cytoplasmic ribonucleoprotein granules, CAPRIN1 mediates recruitment of CNOT7 deadenylase, leading to mRNA deadenylation and degradation. Binds directly and selectively to MYC and CCND2 mRNAs. In neuronal cells, directly binds to several mRNAs associated with RNA granules, including BDNF, CAMK2A, CREB1, MAP2, NTRK2 mRNAs, as well as to GRIN1 and KPNB1 mRNAs, but not to rRNAs[1][2][3][4][5][6][7][8][9][10].
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Subcellular Localization
Cytoplasm, Cytoplasmic ribonucleoprotein granule; Cytoplasm, cytosol; Cell projection, dendrite; Cell projection, lamellipodium
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Expression
Tissue_Specificity: Ubiquitous. -
Isoforms & Post-Translational Modification
Caprin1 has 3 isoforms, Q14444-1: amino acid length is 709, molecular weight is 78366 Da (predicted); Q14444-2: amino acid length is 694, molecular weight is 76862 Da (predicted); Q14444-3: amino acid length is 593, molecular weight is 66227 Da (predicted).
Tyrosine phosphorylation by EPHA4 promotes interaction with FMR1 and liquid-liquid phase separation (LLPS) for the formation of a membraneless compartment that concentrates mRNAs with associated regulatory factors -
Subunit
May form homomultimers.
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SwissProt ID
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Synonyms
GPIAP1, GPIP137, M11S1, RNG105, CAPRIN1, Caprin-1, Cell cycle-associated protein 1, Cytoplasmic activation- and proliferation-associated protein 1, GPI-anchored membrane protein 1, GPI-anchored protein p137, Membrane component chromosome 11 surface marker 1, RNA granule protein 105, GPI-p137, p137GPI
Documentation
[1]. Solomon S, et al. Distinct structural features of caprin-1 mediate its interaction with G3BP-1 and its induction of phosphorylation of eukaryotic translation initiation factor 2alpha, entry to cytoplasmic stress granules, and selective interaction with a subset of mRNAs. Mol Cell Biol. 2007 Mar;27(6):2324-42. [Content Brief]
[2]. Kim TH, et al. Phospho-dependent phase separation of FMRP and CAPRIN1 recapitulates regulation of translation and deadenylation. Science. 2019 Aug 23;365(6455):825-829. [Content Brief]
[3]. Pavinato L, et al. CAPRIN1 haploinsufficiency causes a neurodevelopmental disorder with language impairment, ADHD and ASD. Brain. 2023 Feb 13;146(2):534-548. [Content Brief]
[4]. Jia X, et al. De novo variants in genes regulating stress granule assembly associate with neurodevelopmental disorders. Sci Adv. 2022 Aug 19;8(33):eabo7112. [Content Brief]
[5]. Sanders DW, et al. Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization. Cell. 2020 Apr 16;181(2):306-324.e28. [Content Brief]
[6]. Yang P, et al. G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules. Cell. 2020 Apr 16;181(2):325-345.e28. [Content Brief]
[7]. Guillén-Boixet J, et al. RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation. Cell. 2020 Apr 16;181(2):346-361.e17. [Content Brief]
[8]. Kim TH, et al. Interaction hot spots for phase separation revealed by NMR studies of a CAPRIN1 condensed phase. Proc Natl Acad Sci U S A. 2021 Jun 8;118(23):. [Content Brief]
[9]. Toyama Y, et al. Mapping the per-residue surface electrostatic potential of CAPRIN1 along its phase-separation trajectory. Proc Natl Acad Sci U S A. 2022 Sep 6;119(36):e2210492119. [Content Brief]
[10]. Song D, et al. Yin and yang regulation of stress granules by Caprin-1. Proc Natl Acad Sci U S A. 2022 Nov;119(44):e2207975119. [Content Brief]
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