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CRMP4 Antibody (YA2777)

(Synonyms: CRMP4; DPYSL3; DRP3; LCRMP; TUC4; ULIP1)
Customer Review

Based on 1 Customer Validation

CRMP4 Antibody (YA2777) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CRMP4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CRMP4 Antibody (YA2777)
  • CRMP4 Antibody (YA2777) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:2000 1:50-1:200 1:50

Product Details

Description

CRMP4 Antibody (YA2777) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CRMP4.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 62 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 62 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human CRMP4 aa450-500/570.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CRMP4 Antibody (YA2777)
    Western blot analysis was performed on protein extracts (25 μg) from Mouse brain (lane 2), Rat brain (lane 3) and NIH/3T3 (lane 4) using CRMP4 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    CRMP4 is a Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration

  • Subcellular Localization

    Cytoplasm; Cell projection, growth cone

  • Expression


    Tissue_specificity:Mainly expressed in heart and skeletal muscle. Also strongly expressed in fetal brain and spinal cord

  • Isoforms & Post-Translational Modification

    Q14195 has 2 isomers: Q14195-1: 61963 Da (predicted); Q14195-2: 73911 Da (predicted).
    Phosphorylation on Ser-522 by DYRK2 promotes subsequent phosphorylation on Thr-509, Thr-514 and Ser-518 by GSK3

  • Subunit

    Homotetramer, and heterotetramer with CRMP1, DPYSL2, DPYSL4 or DPYSL5 (By similarity). Interacts with synaptic vesicle protein 2 and SH3A domain of intersectin (By similarity). Interacts with FLNA (PubMed:25358863)

  • SwissProt ID

    Q14195

  • Gene ID
    1809 [NCBI]
  • Synonyms

    CRMP4; DPYSL3; DRP3; LCRMP; TUC4; ULIP1

  • Research Field

    Neuroscience

CRMP4 Antibody (YA2777) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Mouse brain (lane 2), Rat brain (lane 3) and NIH/3T3 (lane 4) using CRMP4 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • CRMP4 Antibody (YA2777) Product Image
    1/16
  • Experimental Validation Results for CRMP4 Antibody (YA2777)
  • CRMP4 Antibody (YA2777) Product Image
  • Experimental Validation Results for CRMP4 Antibody (YA2777)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Mouse brain (lane 2), Rat brain (lane 3) and NIH/3T3 (lane 4) using CRMP4 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for CRMP4 Antibody (YA2777)
  • CRMP4 Antibody (YA2777) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from Mouse brain (lane 2), Rat brain (lane 3) and NIH/3T3 (lane 4) using CRMP4 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • CRMP4 Antibody (YA2777) Product Image WB