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TRIP6 Antibody (YA4290)

(Synonyms: OIP1; OIP-1; ZRP-1; TRIP-6; TRIP6i2)
Customer Review

Based on 1 Customer Validation

TRIP6 Antibody (YA4290) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TRIP6.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL WB FC
  • Experimental Validation Results for TRIP6 Antibody (YA4290)
  • Experimental Validation Results for TRIP6 Antibody (YA4290)
  • Experimental Validation Results for TRIP6 Antibody (YA4290)
  • Experimental Validation Results for TRIP6 Antibody (YA4290)
  • TRIP6 Antibody (YA4290) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:400 1:10000

Product Details

Description

TRIP6 Antibody (YA4290) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TRIP6.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 50 kDa
Immunogen

Purified recombinant fragment of human TRIP6 (AA: 107-291) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718943

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TRIP6 Antibody (YA4290)
    Western blot analysis of extracts from K562 (lane 2(20μg), A431 (lane 3(20μg), Hela (lane 4(20μg), HepG2 (lane 5(20μg) using TRIP6 Antibody（HY-P84593). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TRIP6 Antibody (YA4290)
    Western blot analysis of extracts from A431(lane 1(40μg) )and MCF-7(lane 2(40μg) ) using TRIP6 antibody(HY-P84593). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10
  • Experimental Validation Results for TRIP6 Antibody (YA4290)
    Flow cytometric analysis of 1X10^6 K562 cells labeling TRIP6 Antibody(HY-P84593
  • Experimental Validation Results for TRIP6 Antibody (YA4290)
    Flow cytometric analysis of 1X106 K562 cells labeling TRIP6 Antibody (HY-P84593, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    TRIP6 is a Relays signals from the cell surface to the nucleus to weaken adherens junction and promote actin cytoskeleton reorganization and cell invasiveness. Involved in lysophosphatidic acid-induced cell adhesion and migration. Acts as a transcriptional coactivator for NF-kappa-B and JUN, and mediates the transrepression of these transcription factors induced by glucocorticoid receptor

  • Subcellular Localization

    Cytoplasm, cytoskeleton; Cell junction, focal adhesion; Nucleus; Cytoplasm

  • Expression


    Tissue_specificity:Abundantly expressed in kidney, liver and lung. Lower levels in heart, placenta and pancreas. Expressed in colonic epithelial cells. Up-regulated in colonic tumors

  • Isoforms & Post-Translational Modification

    Q15654 has 3 isomers: Q15654-1: 50288 Da (predicted); Q15654-2: 10602 Da (predicted); Q15654-3: 8277 Da (predicted).
    Phosphorylation at Tyr-55 by SRC is required for enhancement of lysophosphatidic acid-induced cell migration. Tyr-55 is dephosphorylated by PTPN13

  • Subunit

    Specifically interacts with the ligand binding domain of the thyroid receptor (TR) in the presence of thyroid hormone (PubMed:14688263). Interacts (via the third LIM domain and C-terminus) with PTPN13 (via the second PDZ domain) (PubMed:10400701, PubMed:10826496, PubMed:17591779, PubMed:19017743). Interacts (via the second LIM domain or via the third LIM domain plus C-terminus) with PDLIM4 (via PDZ domain) (PubMed:10826496). Found in a complex with PTPN13 and PDLIM4 (By similarity). Interacts with SVIL isoform 2 (PubMed:16880273). Interacts with LPAR2 but not other LPA receptors (PubMed:14688263). Interacts with PRKAA2 (PubMed:16624523). Interacts with MAGI1 (PubMed:19017743). Interacts with SCRIB (PubMed:16137684). In case of infection, interacts with S.typhimurium protein sseI (PubMed:17095609)

  • SwissProt ID

    Q15654

  • Gene ID
    7205 [NCBI]
  • Synonyms

    OIP1; OIP-1; ZRP-1; TRIP-6; TRIP6i2

TRIP6 Antibody (YA4290) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg), A431 (lane 3(20μg), Hela (lane 4(20μg), HepG2 (lane 5(20μg) using TRIP6 Antibody（HY-P84593). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A431(lane 1(40μg) )and MCF-7(lane 2(40μg) ) using TRIP6 antibody(HY-P84593). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 K562 cells labeling TRIP6 Antibody(HY-P84593

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 K562 cells labeling TRIP6 Antibody (HY-P84593, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • TRIP6 Antibody (YA4290) Product Image
    1/16
  • Experimental Validation Results for TRIP6 Antibody (YA4290)
  • Experimental Validation Results for TRIP6 Antibody (YA4290)
  • Experimental Validation Results for TRIP6 Antibody (YA4290)
  • Experimental Validation Results for TRIP6 Antibody (YA4290)
  • TRIP6 Antibody (YA4290) Product Image
  • Experimental Validation Results for TRIP6 Antibody (YA4290)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2(20μg), A431 (lane 3(20μg), Hela (lane 4(20μg), HepG2 (lane 5(20μg) using TRIP6 Antibody（HY-P84593). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TRIP6 Antibody (YA4290)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A431(lane 1(40μg) )and MCF-7(lane 2(40μg) ) using TRIP6 antibody(HY-P84593). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10
  • Experimental Validation Results for TRIP6 Antibody (YA4290)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 K562 cells labeling TRIP6 Antibody(HY-P84593
  • Experimental Validation Results for TRIP6 Antibody (YA4290)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 K562 cells labeling TRIP6 Antibody (HY-P84593, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for TRIP6 Antibody (YA4290)
  • Experimental Validation Results for TRIP6 Antibody (YA4290)
  • Experimental Validation Results for TRIP6 Antibody (YA4290)
  • Experimental Validation Results for TRIP6 Antibody (YA4290)
  • TRIP6 Antibody (YA4290) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 2(20μg), A431 (lane 3(20μg), Hela (lane 4(20μg), HepG2 (lane 5(20μg) using TRIP6 Antibody（HY-P84593). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

  • TRIP6 Antibody (YA4290) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A431(lane 1(40μg) )and MCF-7(lane 2(40μg) ) using TRIP6 antibody(HY-P84593). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10

  • TRIP6 Antibody (YA4290) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 K562 cells labeling TRIP6 Antibody(HY-P84593

  • TRIP6 Antibody (YA4290) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 K562 cells labeling TRIP6 Antibody (HY-P84593, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • TRIP6 Antibody (YA4290) Product Image WB
  • TRIP6 Antibody (YA4290) Product Image WB
  • TRIP6 Antibody (YA4290) Product Image FC
  • TRIP6 Antibody (YA4290) Product Image FC