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Ephrin Receptor B3 Antibody (YA2877)

(Synonyms: EPHB3; ETK2; HEK2; TYRO6; Ephrin type-B receptor 3; EPH-like tyrosine kinase 2; EPH-like kinase 2; Embryonic kinase 2; EK2; hEK2; Tyrosine-protein kinase TYRO6)
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Based on 1 Customer Validation

Ephrin Receptor B3 Antibody (YA2877) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ephrin Receptor B3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Ephrin Receptor B3 Antibody (YA2877)
  • Ephrin Receptor B3 Antibody (YA2877) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Ephrin Receptor B3 Antibody (YA2877) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ephrin Receptor B3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 110 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 110 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Eph receptor B3

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Ephrin Receptor B3 Antibody (YA2877)
    Western blot analysis of extracts from HepG2 (lane2(20μg), Jurkat (lane3(20μg), MOLT-4 (lane4(20μg) and Rat liver tissue (lane5(20μg) using Ephrin Receptor B3 Antibody (HY-P83132). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Ephrin Receptor B3 receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance also plays an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell projection, dendrite

  • Expression


    Tissue_specificity:general expression

  • Subunit

    Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity)

  • SwissProt ID

    P54753

  • Gene ID
    2049 [NCBI]
  • Synonyms

    EPHB3; ETK2; HEK2; TYRO6; Ephrin type-B receptor 3; EPH-like tyrosine kinase 2; EPH-like kinase 2; Embryonic kinase 2; EK2; hEK2; Tyrosine-protein kinase TYRO6

  • Research Field

    Cardiovascular

Ephrin Receptor B3 Antibody (YA2877) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane2(20μg), Jurkat (lane3(20μg), MOLT-4 (lane4(20μg) and Rat liver tissue (lane5(20μg) using Ephrin Receptor B3 Antibody (HY-P83132). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • Ephrin Receptor B3 Antibody (YA2877) Product Image
    1/16
  • Experimental Validation Results for Ephrin Receptor B3 Antibody (YA2877)
  • Ephrin Receptor B3 Antibody (YA2877) Product Image
  • Experimental Validation Results for Ephrin Receptor B3 Antibody (YA2877)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane2(20μg), Jurkat (lane3(20μg), MOLT-4 (lane4(20μg) and Rat liver tissue (lane5(20μg) using Ephrin Receptor B3 Antibody (HY-P83132). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Ephrin Receptor B3 Antibody (YA2877)
  • Ephrin Receptor B3 Antibody (YA2877) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane2(20μg), Jurkat (lane3(20μg), MOLT-4 (lane4(20μg) and Rat liver tissue (lane5(20μg) using Ephrin Receptor B3 Antibody (HY-P83132). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • Ephrin Receptor B3 Antibody (YA2877) Product Image WB