Wilms Tumor Protein Antibody (YA3856)
(Synonyms: GUD; AWT1; WAGR; WT33; NPHS4; WIT-2)Wilms Tumor Protein Antibody (YA3856) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Wilms Tumor Protein.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, IHC-P, ICC/IF, FC, ELISA
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Reactivity :
Human, Monkey
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:1000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
Wilms Tumor Protein Antibody (YA3856) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Wilms Tumor Protein.
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Host Mouse
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Species ReactivityHuman, Monkey
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Observed Molecular WeightObserved band size: 49 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 49 kDa
Purified recombinant fragment of human WT1 (AA: 1-181) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS with 0.05% sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Wilms Tumor Protein transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA[1][2][3][4][5][6].
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Subcellular Localization
Nucleus; Nucleus, nucleolus; Cytoplasm; Nucleus speckle; Nucleus, nucleoplasm
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Expression
Tissue_specificity:It is expressed in the kidneys and some hematopoietic cells. -
Isoforms & Post-Translational Modification
P19544 has 8 isomers: P19544-1: 49188 Da (predicted); P19544-2: 47195 Da (predicted); P19544-3: 47511 Da (predicted); P19544-4: 48872 Da (predicted); P19544-6: 34447 Da (predicted); P19544-7: 56883 Da (predicted); P19544-8: 55206 Da (predicted); P19544-9: 33086 Da (predicted).
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Subunit
Homodimer. Interacts with WTIP. Interacts with actively translating polysomes. Detected in nuclear ribonucleoprotein (mRNP) particles. Interacts with HNRNPU via the zinc-finger region. Interacts with U2AF2. Interacts with CITED2 (By similarity). Interacts with ZNF224 via the zinc-finger region. Interacts with WTAP and SRY. Interacts with AMER1. Interacts with RBM4
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SwissProt ID
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Synonyms
GUD; AWT1; WAGR; WT33; NPHS4; WIT-2
Documentation
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