Wilms Tumor Protein Antibody (YA3856)

Cat. No.: HY-P84159: Data Sheet User Guide for Antibodies
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Wilms Tumor Protein Antibody (YA3856) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Wilms Tumor Protein.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

Wilms Tumor Protein Antibody (YA3856) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Wilms Tumor Protein.

Host

Mouse

Molecular Weight

Predicted band size: 49 kDa;

Observed band size: 49 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Monkey

SwissProt ID

P19544

Gene ID

7490 [NCBI]

Immunogen

Purified recombinant fragment of human WT1 (AA: 1-181) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:17716689, PubMed:25258363, PubMed:7862533). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921)

Subcellular Localization：Nucleus; Nucleus, nucleolus; Cytoplasm; Nucleus speckle; Nucleus, nucleoplasm

Expression：
Tissue_specificity:It is expressed in the kidneys and some hematopoietic cells.

Isoforms & Post-Translational Modification：P19544 has 8 isomers: P19544-1: 49188 Da (predicted); P19544-2: 47195 Da (predicted); P19544-3: 47511 Da (predicted); P19544-4: 48872 Da (predicted); P19544-6: 34447 Da (predicted); P19544-7: 56883 Da (predicted); P19544-8: 55206 Da (predicted); P19544-9: 33086 Da (predicted).

Subunit：Homodimer. Interacts with WTIP. Interacts with actively translating polysomes. Detected in nuclear ribonucleoprotein (mRNP) particles. Interacts with HNRNPU via the zinc-finger region. Interacts with U2AF2. Interacts with CITED2 (By similarity). Interacts with ZNF224 via the zinc-finger region. Interacts with WTAP and SRY. Interacts with AMER1. Interacts with RBM4

Synonyms

GUD; AWT1; WAGR; WT33; NPHS4; WIT-2

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/sk-br-3-cell.html [Content Brief]