Copy Link Print Information

MPO Antibody (YA5574)

(Synonyms: 84 kDa myeloperoxidase antibody; 89 kDa myeloperoxidase antibody; EC1.11.2.2 antibody; fj80f04 antibody; MPO antibody; mpx antibody; myeloid-specific peroxidase antibody; Myeloperoxidase antibody; Myeloperoxidase heavy chain antibody; Myeloperoxidase light chain antibody; PERM_HUMAN antibody; wu:fj80f04 antibody)

MPO Antibody (YA5574) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to MPO.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    IHC-P, WB, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MPO Antibody (YA5574)
  • Experimental Validation Results for MPO Antibody (YA5574)
  • MPO Antibody (YA5574) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1000 1:500-2000 1:100-500 1:1000-5000

Product Details

Description

MPO Antibody (YA5574) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to MPO.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 84 kDa
Immunogen

Synthesized peptide derived from human Myeloperoxidase(MPO) AA range: 350-450

Purification

Protein G

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MPO Antibody (YA5574)
    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Myeloperoxidase(MPO) (PTR2562) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MPO Antibody (YA5574)
    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Myeloperoxidase(MPO) (PTR2562) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    MPO is a Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage[1][2].

  • Subcellular Localization

    Lysosome

  • Isoforms & Post-Translational Modification

    P05164 has 3 isomers: P05164-1: 83869 Da (predicted); P05164-2: 73854 Da (predicted); P05164-3: 87248 Da (predicted).

  • Subunit

    Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Found in a complex with CP and LTF; interacts directly with CP, which protects CP antioxidant properties by MPO (PubMed:23843990)

  • SwissProt ID

    P05164

  • Gene ID
    4353 [NCBI]
  • Synonyms

    84 kDa myeloperoxidase antibody; 89 kDa myeloperoxidase antibody; EC1.11.2.2 antibody; fj80f04 antibody; MPO antibody; mpx antibody; myeloid-specific peroxidase antibody; Myeloperoxidase antibody; Myeloperoxidase heavy chain antibody; Myeloperoxidase light chain antibody; PERM_HUMAN antibody; wu:fj80f04 antibody

MPO Antibody (YA5574) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Myeloperoxidase(MPO) (PTR2562) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Myeloperoxidase(MPO) (PTR2562) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MPO Antibody (YA5574) Product Image
    1/16
  • Experimental Validation Results for MPO Antibody (YA5574)
  • Experimental Validation Results for MPO Antibody (YA5574)
  • MPO Antibody (YA5574) Product Image
  • Experimental Validation Results for MPO Antibody (YA5574)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Myeloperoxidase(MPO) (PTR2562) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MPO Antibody (YA5574)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Myeloperoxidase(MPO) (PTR2562) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MPO Antibody (YA5574)
  • Experimental Validation Results for MPO Antibody (YA5574)
  • MPO Antibody (YA5574) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Myeloperoxidase(MPO) (PTR2562) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MPO Antibody (YA5574) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Myeloperoxidase(MPO) (PTR2562) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MPO Antibody (YA5574) Product Image IHC-P
  • MPO Antibody (YA5574) Product Image IHC-P