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Cx40/GJA5 Antibody (YA6895)

(Synonyms: Connexin 40 antibody)
Customer Review

Based on 1 Customer Validation

Cx40/GJA5 Antibody (YA6895) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cx40/GJA5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cx40/GJA5 Antibody (YA6895)
  • Cx40/GJA5 Antibody (YA6895) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:2000 1-2μg/sample

Product Details

Description

Cx40/GJA5 Antibody (YA6895) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cx40/GJA5.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 40 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 40 kDa
Immunogen

Recombinant protein within human GJA5 aa 227-358.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cx40/GJA5 Antibody (YA6895)
    Western blot analysis of extracts from NIH-3T3 (lane 1(20μg)) 、Mouse lung (lane 2(40μg)) and Mouse heart (lane 3(40μg)) using Cx40/GJA5 Antibody (HY-P87207) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Cx40/GJA5 is an One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell

  • Subcellular Localization

    Cell membrane,Cell junction, gap junction

  • Isoforms & Post-Translational Modification

    P36382: 358 amino acids, molecular weight 40380 Da.

  • Subunit

    A connexon is composed of a hexamer of connexins

  • SwissProt ID

    P36382

  • Synonyms

    Connexin 40 antibody

Cx40/GJA5 Antibody (YA6895) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from NIH-3T3 (lane 1(20μg)) 、Mouse lung (lane 2(40μg)) and Mouse heart (lane 3(40μg)) using Cx40/GJA5 Antibody (HY-P87207) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Cx40/GJA5 Antibody (YA6895) Product Image
    1/16
  • Experimental Validation Results for Cx40/GJA5 Antibody (YA6895)
  • Cx40/GJA5 Antibody (YA6895) Product Image
  • Experimental Validation Results for Cx40/GJA5 Antibody (YA6895)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from NIH-3T3 (lane 1(20μg)) 、Mouse lung (lane 2(40μg)) and Mouse heart (lane 3(40μg)) using Cx40/GJA5 Antibody (HY-P87207) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cx40/GJA5 Antibody (YA6895)
  • Cx40/GJA5 Antibody (YA6895) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from NIH-3T3 (lane 1(20μg)) 、Mouse lung (lane 2(40μg)) and Mouse heart (lane 3(40μg)) using Cx40/GJA5 Antibody (HY-P87207) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Cx40/GJA5 Antibody (YA6895) Product Image WB