Cx40/GJA5 Antibody (YA6895)

Cat. No.: HY-P87207: Ficha de datos COA
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Cx40/GJA5 Antibody (YA6895) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cx40/GJA5.

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Tamaño	Stock	Cantidad
10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Descripciòn

Descripciòn

Cx40/GJA5 Antibody (YA6895) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cx40/GJA5.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Peso molecular

Predicted band size: 40 kDa;

Observed band size: 40 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Monkey

SwissProt ID

P36382

Immunogen

Recombinant protein within human GJA5 aa 227-358.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000
IP IP: Immunoprecipitation	1-2μg/sample

Pureza	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from NIH-3T3 (lane 1(20μg)) 、Mouse lung (lane 2(40μg)) and Mouse heart (lane 3(40μg)) using Cx40/GJA5 Antibody (HY-P87207) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell

Subcellular Localization：Cell membrane,Cell junction, gap junction

Isoforms & Post-Translational Modification：P36382: 358 amino acids, molecular weight 40380 Da.

Subunit：A connexon is composed of a hexamer of connexins

Synonyms

Connexin 40 antibody

Documentación

Select Batch:

Ficha de datos (231 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)