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MAVS Antibody (YA6648)

(Synonyms: CARD adapter inducing interferon beta antibody; CARD adaptor inducing IFN beta antibody; Cardif antibody; DKFZp666M015 antibody; FLJ27482 antibody; FLJ41962 antibody; IFN B promoter stimulator 1 antibody; Interferon beta promoter stimulator protein 1 antibody; Ips 1 antibody; IPS-1 antibody; CARD adapter inducing interferon beta antibody; CARD adaptor inducing IFN beta antibody)
Customer Review

Based on 1 Customer Validation

MAVS Antibody (YA6648) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MAVS.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for MAVS Antibody (YA6648)
  • Experimental Validation Results for MAVS Antibody (YA6648)
  • Experimental Validation Results for MAVS Antibody (YA6648)
  • Experimental Validation Results for MAVS Antibody (YA6648)
  • Experimental Validation Results for MAVS Antibody (YA6648)
  • MAVS Antibody (YA6648) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000 1:200-1:1000 1:100 1-2μg/sample

Product Details

Description

MAVS Antibody (YA6648) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MAVS.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 75 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 56 kDa
Immunogen

Synthetic peptide within human MAVS aa 2-50.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719234

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MAVS Antibody (YA6648)
    Western blot analysis of extracts from MCF-7(lane 2(20ug) , C2C12(lane 3(20ug) and THP-1(lane 4(20ug) using MAVS Antibody (HY-P86955) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MAVS Antibody (YA6648)
    Immunohistochemical analysis of paraffin-embedded mouse heart tissue using MAVS Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MAVS Antibody (YA6648)
    Immunohistochemical analysis of paraffin-embedded mouse heart tissue using MAVS Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MAVS Antibody (YA6648)
    Immunocytochemistry analysis of MCF-7 cells labeling MAVS with MAVS Antibody (YA6648) (HY-P86955) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MAVS Antibody (YA6648) ((HY-P86955) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MAVS Antibody (YA6648)
    Immunocytochemistry analysis of A431 cells labeling MAVS with MAVS Antibody (YA6648) (HY-P86955) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MAVS Antibody (YA6648) ((HY-P86955) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    MAVS is an Adapter required for innate immune defense against viruses. Acts downstream of DHX33, RIGI and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFNB and RANTES (CCL5). Peroxisomal and mitochondrial MAVS act sequentially to create an antiviral cellular state. Upon viral infection, peroxisomal MAVS induces the rapid interferon-independent expression of defense factors that provide short-term protection, whereas mitochondrial MAVS activates an interferon-dependent signaling pathway with delayed kinetics, which amplifies and stabilizes the antiviral response. May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis. Involved in NLRP3 inflammasome activation by mediating NLRP3 recruitment to mitochondria[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17].

  • Subcellular Localization

    Mitochondrion outer membrane,Mitochondrion,Peroxisome

  • Expression


    Tissue_Specificity: Present in T-cells, monocytes, epithelial cells and hepatocytes (at protein level). Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes

  • Isoforms & Post-Translational Modification

    Q7Z434 has 6 isomers: Q7Z434-1: 56528 Da (predicted); Q7Z434-2: 15996 Da (predicted); Q7Z434-3: 17043 Da (predicted); Q7Z434-4: 40468 Da (predicted); Q7Z434-5: 14385 Da (predicted); Q7Z434-6: 15012 Da (predicted).
    Following activation, phosphorylated by TBK1 at Ser-442 in the pLxIS motif (PubMed:25636800, PubMed:27302953)丨Ubiquitinated (PubMed:19881509, PubMed:23087404, PubMed:25636800, PubMed:38016475)丨Palmitoylated by ZHDDC4丨Proteolytically cleaved by apoptotic caspases during apoptosis, leading to its inactivation (PubMed:30878284)丨(Microbial infection) Cleaved and degraded by hepatitis A virus (HAV) protein 3ABC allowing the virus to disrupt the activation of host IRF3 through the MDA5 pathway丨(Microbial infection) Cleaved by the protease 2A of coxsackievirus B3, poliovirus and enterovirus 71 allowing the virus to disrupt the host type I interferon production丨(Microbial infection) Cleaved by Seneca Valley virus protease 3C allowing the virus to suppress interferon type-I production丨(Microbial infection) Cleaved by HCV protease NS3/4A, thereby preventing the establishment of an antiviral state丨(Microbial infection) UFMylated by ULF1 in association with Epstein-Barr virus BILF1; leading to MAVS routing to the lysosome

  • Subunit

    Self-associates and polymerizes (via CARD domains) to form 400 nM long three-stranded helical filaments on mitochondria, filament nucleation requires interaction with RIGI whose CARD domains act as a template for filament assembly (PubMed:24569476, PubMed:25018021, PubMed:27992402, PubMed:26246171)

  • SwissProt ID

    Q7Z434

  • Gene ID
    57506 [NCBI]
  • Synonyms

    CARD adapter inducing interferon beta antibody; CARD adaptor inducing IFN beta antibody; Cardif antibody; DKFZp666M015 antibody; FLJ27482 antibody; FLJ41962 antibody; IFN B promoter stimulator 1 antibody; Interferon beta promoter stimulator protein 1 antibody; Ips 1 antibody; IPS-1 antibody; CARD adapter inducing interferon beta antibody; CARD adaptor inducing IFN beta antibody

MAVS Antibody (YA6648) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20ug) , C2C12(lane 3(20ug) and THP-1(lane 4(20ug) using MAVS Antibody (HY-P86955) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse heart tissue using MAVS Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse heart tissue using MAVS Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling MAVS with MAVS Antibody (YA6648) (HY-P86955) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MAVS Antibody (YA6648) ((HY-P86955) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling MAVS with MAVS Antibody (YA6648) (HY-P86955) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MAVS Antibody (YA6648) ((HY-P86955) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MAVS Antibody (YA6648) Product Image
    1/16
  • Experimental Validation Results for MAVS Antibody (YA6648)
  • Experimental Validation Results for MAVS Antibody (YA6648)
  • Experimental Validation Results for MAVS Antibody (YA6648)
  • Experimental Validation Results for MAVS Antibody (YA6648)
  • Experimental Validation Results for MAVS Antibody (YA6648)
  • MAVS Antibody (YA6648) Product Image
  • Experimental Validation Results for MAVS Antibody (YA6648)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20ug) , C2C12(lane 3(20ug) and THP-1(lane 4(20ug) using MAVS Antibody (HY-P86955) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for MAVS Antibody (YA6648)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse heart tissue using MAVS Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MAVS Antibody (YA6648)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse heart tissue using MAVS Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for MAVS Antibody (YA6648)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling MAVS with MAVS Antibody (YA6648) (HY-P86955) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MAVS Antibody (YA6648) ((HY-P86955) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MAVS Antibody (YA6648)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling MAVS with MAVS Antibody (YA6648) (HY-P86955) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MAVS Antibody (YA6648) ((HY-P86955) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for MAVS Antibody (YA6648)
  • Experimental Validation Results for MAVS Antibody (YA6648)
  • Experimental Validation Results for MAVS Antibody (YA6648)
  • Experimental Validation Results for MAVS Antibody (YA6648)
  • Experimental Validation Results for MAVS Antibody (YA6648)
  • MAVS Antibody (YA6648) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from MCF-7(lane 2(20ug) , C2C12(lane 3(20ug) and THP-1(lane 4(20ug) using MAVS Antibody (HY-P86955) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • MAVS Antibody (YA6648) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue using MAVS Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MAVS Antibody (YA6648) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue using MAVS Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • MAVS Antibody (YA6648) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling MAVS with MAVS Antibody (YA6648) (HY-P86955) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MAVS Antibody (YA6648) ((HY-P86955) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MAVS Antibody (YA6648) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling MAVS with MAVS Antibody (YA6648) (HY-P86955) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MAVS Antibody (YA6648) ((HY-P86955) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • MAVS Antibody (YA6648) Product Image WB
  • MAVS Antibody (YA6648) Product Image IHC-P
  • MAVS Antibody (YA6648) Product Image IHC-P
  • MAVS Antibody (YA6648) Product Image ICC
  • MAVS Antibody (YA6648) Product Image ICC