Copy Link Print Information

Glucose Transporter GLUT4 Antibody (YA753)

(Synonyms: Glut4, Slc2a4, GT2, GLUT-4)
1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

Glucose Transporter GLUT4 Antibody (YA753) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Glucose Transporter GLUT4.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ICC/IF, IHC-P

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
  • Glucose Transporter GLUT4 Antibody (YA753) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) Glucose Transporter GLUT4 Antibody (YA753)

More

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:2000-1:5000 1:100-1:200 1:100-1:200

Product Details

Description

Glucose Transporter GLUT4 Antibody (YA753) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Glucose Transporter GLUT4.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 55 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Glucose Transporter GLUT4.AA range:460-509.

Sensitivity

Endogenous

Purification

Immunogen affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3102568

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
    Western blot analysis of extracts from Mouse heart tissue (lane2(20μg), Hela (lane3(20μg), SK-OV-3 (lane4(20μg) and HEK293 (lane5(20μg) using Glucose Transporter GLUT4 Antibody (HY-P80495). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
    Immunohistochemical analysis of paraffin-embedded mouse heart tissue using Glucose Transporter GLUT4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
    Immunohistochemical analysis of paraffin-embedded mouse heart tissue using Glucose Transporter GLUT4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
    Immunocytochemistry analysis of HeLa cells labeling Glucose Transporter GLUT4 with Glucose Transporter GLUT4 Antibody (HY-P80495) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glucose Transporter GLUT4 Antibody (HY-P80495) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
    Immunocytochemistry analysis of HepG2 cells labeling Glucose Transporter GLUT4 with Glucose Transporter GLUT4 Antibody (HY-P80495) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glucose Transporter GLUT4 Antibody (HY-P80495) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Glucose Transporter GLUT4 plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcriptional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be involved in fat oxidation and non-oxidative glucose metabolism and in the regulation of energy expenditure. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:It is widely distributed throughout the body, with higher expression in the heart, brain, and skeletal muscle.

    Induction:Repressed by saturated fatty acids such as palmitate and stearate in skeletal muscle cells. Induced by insulin and reduced by aging in skeletal muscle biopsies. Down-regulated in type 2 diabetes mellitus subjects as well as in pre-diabetics

  • Isoforms & Post-Translational Modification

    Q86YN6 has 6 isomers: Q86YN6-1: 113222 Da (predicted); Q86YN6-2: 110981 Da (predicted); Q86YN6-3: 112568 Da (predicted); Q86YN6-4: 110327 Da (predicted); Q86YN6-5: 108919 Da (predicted); Q86YN6-6: 106230 Da (predicted).

  • Subunit

    Interacts with hepatocyte nuclear factor 4-alpha/HNF4A, Sterol regulatory binding transcription factor 1/SREBF1, PPAR-alpha/PPARA, thyroid hormone receptor beta/THRB and host cell factor/HCFC1. Interacts with estrogen-related receptor gamma/ESRRG and alpha/ESRRA. Interacts with PRDM16 (By similarity). Interacts with estrogen receptor alpha/ESR1

  • SwissProt ID

    P14672

  • Gene ID
    6517 [NCBI]
  • Synonyms

    Glut4, Slc2a4, GT2, GLUT-4

  • Research Field

    Signal Transduction

Glucose Transporter GLUT4 Antibody (YA753) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Mouse heart tissue (lane2(20μg), Hela (lane3(20μg), SK-OV-3 (lane4(20μg) and HEK293 (lane5(20μg) using Glucose Transporter GLUT4 Antibody (HY-P80495). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse heart tissue using Glucose Transporter GLUT4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse heart tissue using Glucose Transporter GLUT4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling Glucose Transporter GLUT4 with Glucose Transporter GLUT4 Antibody (HY-P80495) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glucose Transporter GLUT4 Antibody (HY-P80495) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Glucose Transporter GLUT4 with Glucose Transporter GLUT4 Antibody (HY-P80495) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glucose Transporter GLUT4 Antibody (HY-P80495) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Glucose Transporter GLUT4 Antibody (YA753) Product Image
    1/16
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
  • Glucose Transporter GLUT4 Antibody (YA753) Product Image
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Mouse heart tissue (lane2(20μg), Hela (lane3(20μg), SK-OV-3 (lane4(20μg) and HEK293 (lane5(20μg) using Glucose Transporter GLUT4 Antibody (HY-P80495). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse heart tissue using Glucose Transporter GLUT4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse heart tissue using Glucose Transporter GLUT4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling Glucose Transporter GLUT4 with Glucose Transporter GLUT4 Antibody (HY-P80495) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glucose Transporter GLUT4 Antibody (HY-P80495) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Glucose Transporter GLUT4 with Glucose Transporter GLUT4 Antibody (HY-P80495) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glucose Transporter GLUT4 Antibody (HY-P80495) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
  • Experimental Validation Results for Glucose Transporter GLUT4 Antibody (YA753)
  • Glucose Transporter GLUT4 Antibody (YA753) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Mouse heart tissue (lane2(20μg), Hela (lane3(20μg), SK-OV-3 (lane4(20μg) and HEK293 (lane5(20μg) using Glucose Transporter GLUT4 Antibody (HY-P80495). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • Glucose Transporter GLUT4 Antibody (YA753) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue using Glucose Transporter GLUT4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Glucose Transporter GLUT4 Antibody (YA753) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue using Glucose Transporter GLUT4 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Glucose Transporter GLUT4 Antibody (YA753) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling Glucose Transporter GLUT4 with Glucose Transporter GLUT4 Antibody (HY-P80495) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glucose Transporter GLUT4 Antibody (HY-P80495) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Glucose Transporter GLUT4 Antibody (YA753) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Glucose Transporter GLUT4 with Glucose Transporter GLUT4 Antibody (HY-P80495) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glucose Transporter GLUT4 Antibody (HY-P80495) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Glucose Transporter GLUT4 Antibody (YA753) Product Image WB
  • Glucose Transporter GLUT4 Antibody (YA753) Product Image IHC-P
  • Glucose Transporter GLUT4 Antibody (YA753) Product Image IHC-P
  • Glucose Transporter GLUT4 Antibody (YA753) Product Image ICC
  • Glucose Transporter GLUT4 Antibody (YA753) Product Image ICC