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CD70 Antibody (YA1409)

(Synonyms: CD70; CD27LG; TNFSF7; TNFSF7G; CD27L)
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Based on 1 Customer Validation

CD70 Antibody (YA1409) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD70.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD70 Antibody (YA1409)
  • CD70 Antibody (YA1409) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:1000-2000 1:50-1:100 1:10000

Product Details

Description

CD70 Antibody (YA1409) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD70.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 30 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 21 kDa
Species Reactivity Database

Entrez Gene: 970 Human

SwissProt: P32970 Human

Immunogen

Recombinant human CD70 protein

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103773

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD70 Antibody (YA1409)
    Western blot analysis of extracts from HUVEC (lane 2(20μg), HUVEC (lane 3(40μg), using CD70 Antibody (YA1409). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    CD70 is expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells. The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells[1][2][3].

  • Subcellular Localization

    Cell membrane; Single-pass type II membrane protein

  • Isoforms & Post-Translational Modification

    P32970 has 2 isomers: P32970-1: 21118 Da (predicted); P32970-2: 23438 Da (predicted).
    N-glycosylated

  • Subunit

    Homotrimer

  • SwissProt ID

    P32970

  • Gene ID
    970 [NCBI]
  • Synonyms

    CD70; CD27LG; TNFSF7; TNFSF7G; CD27L

  • Research Field

    Immunology

CD70 Antibody (YA1409) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HUVEC (lane 2(20μg), HUVEC (lane 3(40μg), using CD70 Antibody (YA1409). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CD70 Antibody (YA1409) Product Image
    1/16
  • Experimental Validation Results for CD70 Antibody (YA1409)
  • CD70 Antibody (YA1409) Product Image
  • Experimental Validation Results for CD70 Antibody (YA1409)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HUVEC (lane 2(20μg), HUVEC (lane 3(40μg), using CD70 Antibody (YA1409). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD70 Antibody (YA1409)
  • CD70 Antibody (YA1409) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HUVEC (lane 2(20μg), HUVEC (lane 3(40μg), using CD70 Antibody (YA1409). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CD70 Antibody (YA1409) Product Image WB