Nucleoside Phosphorylase Antibody
(Synonyms: FLJ94043; FLJ97288; FLJ97312; Inosine phosphorylase; Inosine-guanosine phosphorylase; MGC117396; MGC125915; MGC125916; NP; Np1)
Based on 1 Customer Validation
Nucleoside Phosphorylase Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Nucleoside Phosphorylase.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in TBS (pH7.4), 0.2% BSA, 50% Glycerol, 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:250 | 1:200-1000 | 1:1000 |
Product Details
Nucleoside Phosphorylase Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Nucleoside Phosphorylase.
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Host Rabbit
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 32 kDa
Recombinant protein within human Nucleoside Phosphorylase aa 1-200.
Endogenous
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in TBS (pH7.4), 0.2% BSA, 50% Glycerol, 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Nucleoside Phosphorylase catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Preferentially acts on 6-oxopurine nucleosides including inosine and guanosine[1][2][3].
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Subcellular Localization
Cytoplasm, cytosol
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Expression
Tissue_Specificity: Expressed in red blood cells; overexpressed in red blood cells (cytoplasm) of patients with hereditary non-spherocytic hemolytic anemia of unknown etiology. -
Isoforms & Post-Translational Modification
Nucleoside Phosphorylase has an amino acid length of 289, molecular weight is 32118 Da.
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Subunit
Homotrimer
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SwissProt ID
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Synonyms
FLJ94043; FLJ97288; FLJ97312; Inosine phosphorylase; Inosine-guanosine phosphorylase; MGC117396; MGC125915; MGC125916; NP; Np1
Documentation
[1]. Suarez J, et al. Catalytic site conformations in human PNP by 19F-NMR and crystallography. Chem Biol. 2013 Feb 21;20(2):212-22. [Content Brief]
[2]. Williams SR, et al. A human purine nucleoside phosphorylase deficiency caused by a single base change. J Biol Chem. 1987 Feb 15;262(5):2332-8. [Content Brief]
[3]. Stoeckler JD, et al. Purine nucleoside phosphorylase. 3. Reversal of purine base specificity by site-directed mutagenesis. Biochemistry. 1997 Sep 30;36(39):11749-56. [Content Brief]
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