Perilipin-1 Antibody (YA2538)

Cat. No.: HY-P82793: Data Sheet COA
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User Guide for Antibodies
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Perilipin-1 Antibody (YA2538) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Perilipin-1.

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10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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Perilipin-1 Antibody (YA2538) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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Perilipin-1 Antibody (YA2538) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Perilipin-1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

분자량

Predicted band size: 56 kDa;

Observed band size: 62 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

O60240

Gene ID

5346 [NCBI]

Immunogen

A synthesized peptide derived from human Perilipin A aa475-500.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from MCF-7 (lane 2(20μg), MCF-7 (lane 3(40μg), using Perilipin-1 Antibody (YA2538). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells labeling Perilipin-1 with Perilipin-1 antibody (HY-P82793) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Perilipin-1 antibody (HY-P82793) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of HeLa cells labeling Perilipin-1 with Perilipin-1 antibody (HY-P82793) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Perilipin-1 antibody (HY-P82793) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels

Subcellular Localization：Endoplasmic reticulum; Lipid droplet

Expression：
Tissue_specificity:Protein levels were detected in adipocytes of white adipose tissue (PubMed: 27832861) . It was also detected in visceral adipose tissue and mammary glands (PubMed: 9521880) .

Subunit：Interacts with ABHD5 (By similarity). Interacts with CIDEC (PubMed:23399566). Interacts with AQP7 (PubMed:27832861)

RRID

AB_3104889

Database

Entrez Gene: 5346 Human ; 103968 Mouse ;

SwissProt: O60240 Human ; Q8CGN5 Mouse ;

OMIM: 613877 Human

Research Field

Cardiovascular

Synonyms

Lipid droplet associated protein; PERI; Perilipin-1; PerilipinA; LIN1

각종 서류

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Perilipin-1 Antibody (YA2538) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Perilipin-1 Antibody (YA2538)

Perilipin-1 Antibody (YA2538) Featured Recommendations:

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Perilipin-1 Antibody (YA2538) Related Classifications

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