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  5. Liver Carboxylesterase 1 Antibody (YA2328)

Liver Carboxylesterase 1 Antibody (YA2328)

Cat. No.: HY-P82583
COA
SDS
User Guide for Antibodies Technical Support

Liver Carboxylesterase 1 Antibody (YA2328) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Liver Carboxylesterase 1.

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10 μL 해외재고보유
50 μL 해외재고보유
100 μL 해외재고보유
250 μL   견적 받기  
or 대량구매 문의

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

  • Background

  • 제품 설명

  • References

제품 설명

Liver Carboxylesterase 1 Antibody (YA2328) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Liver Carboxylesterase 1.
Host

Rabbit
Clonality

Recombinant, Monoclonal
분자량
Predicted band size: 63 kDa;
Observed band size: 55 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human Liver Carboxylesterase 1 aa64-82.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IP
IP: Immunoprecipitation
1:40
FC
FC: Flow Cytometry
1:50-1:100
Sensitivity Endogenous Purity Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
선적

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from U937(lane 2(20ug) and U937(lane 3(40ug)using Liver Carboxylesterase 1 Antibody (HY-P82583) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs (PubMed:18762277, PubMed:7980644, PubMed:9169443, PubMed:9490062). Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester (PubMed:18762277, PubMed:7980644, PubMed:9169443, PubMed:9490062). Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine (PubMed:7980644). Catalyzes the transesterification of cocaine to form cocaethylene (PubMed:7980644). Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate (PubMed:7980644). Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes of 2-arachidonoylglycerol and prostaglandins (PubMed:21049984). Hydrolyzes cellular cholesteryl esters to free cholesterols and promotes reverse cholesterol transport (RCT) by facilitating both the initial and final steps in the process (PubMed:11015575, PubMed:16024911, PubMed:16971496, PubMed:18762277). First of all, allows free cholesterol efflux from macrophages to extracellular cholesterol acceptors and secondly, releases free cholesterol from lipoprotein-delivered cholesteryl esters in the liver for bile acid synthesis or direct secretion into the bile (PubMed:16971496, PubMed:18599737, PubMed:18762277)
Subcellular Localization：Endoplasmic reticulum lumen; Cytoplasm; Lipid droplet
Expression：
Tissue_specificity:Expressed predominantly in liver with lower levels in heart and lung (PubMed:10562416) . Expressed in macrophages (PubMed:11015575, PubMed:18762277, PubMed:21049984)
Isoforms & Post-Translational Modification：P23141 has 3 isomers: P23141-1: 62521 Da (predicted); P23141-2: 62592 Da (predicted); P23141-3: 62393 Da (predicted).
Contains sialic acid;Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19
Subunit：Homotrimer and homohexamer. Binds to beta-glucuronidase
RRID
Database

Entrez Gene: 1066 Human ; 12623 Mouse ; 24346 Rat 29225

SwissProt: P23141 Human ; Q8VCC2 Mouse ; P10959 Rat Q63108

OMIM: 114835 Human

Research Field

Cardiovascular
Synonyms
ACAT; CE 1; CEH; CES1; CES2; CESDD1; Egasyn; ES-HTEL; ES-x; Es22; Esterase 22; hCE 1; HMSE; HMSE1; REH; SES1; TGH; Triacylglycerol hydrolase
각종 서류
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)
References

Liver Carboxylesterase 1 Antibody (YA2328) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:
Liver Carboxylesterase 1 Antibody (YA2328)
Cat. No.:
HY-P82583
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