CD28 Antibody (YA9780)
(Synonyms: CD28, T-cell-specific surface glycoprotein CD28, Cd28)CD28 Antibody (YA9780) is a Rabbit-derived and non-conjugated IgG Monoclonal antibody, targeting to CD28.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
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|---|---|---|---|---|
| Dilution Ratio | 1:1000 | 1:200 | 1:100 | 1:20 |
Product Details
CD28 Antibody (YA9780) is a Rabbit-derived and non-conjugated IgG Monoclonal antibody, targeting to CD28.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 40-60 kDa;
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CD28 is a Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis. Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells. Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets. Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells[1][2][3][4][5][6][7].
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Subcellular Localization
Cell membrane
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Expression
Tissue_Specificity: Expressed in T-cells and plasma cells, but not in less mature B-cells. -
Isoforms & Post-Translational Modification
CD28 has 7 isoforms, P10747-1: amino acid length is 220, molecular weight is 25066 Da (predicted); P10747-2: amino acid length is 101, molecular weight is 11528 Da (predicted); P10747-3: amino acid length is 136, molecular weight is 15369 Da (predicted); P10747-4: amino acid length is 123, molecular weight is 14014 Da (predicted); P10747-5: amino acid length is 55, molecular weight is 6099 Da (predicted); P10747-6: amino acid length is 80, molecular weight is 8823 Da (predicted); P10747-7: amino acid length is 234, molecular weight is 26186 Da (predicted).Phosphorylated by LCK (PubMed:7568038). Dephosphorylated by PTPN11 (PubMed:36114179)
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Subunit
Homodimer; disulfide-linked.
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SwissProt ID
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Synonyms
CD28, T-cell-specific surface glycoprotein CD28, Cd28
Documentation
[1]. Gimmi CD, et al. B-cell surface antigen B7 provides a costimulatory signal that induces T cells to proliferate and secrete interleukin 2. Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6575-9. [Content Brief]
[2]. Raab M, et al. p56Lck and p59Fyn regulate CD28 binding to phosphatidylinositol 3-kinase, growth factor receptor-bound protein GRB-2, and T cell-specific protein-tyrosine kinase ITK: implications for T-cell costimulation. Proc Natl Acad Sci U S A. 1995 Sep 12;92(19):8891-5. [Content Brief]
[3]. Skånland SS, et al. T-cell co-stimulation through the CD2 and CD28 co-receptors induces distinct signalling responses. Biochem J. 2014 Jun 15;460(3):399-410. [Content Brief]
[4]. Collins AV, et al. The interaction properties of costimulatory molecules revisited. Immunity. 2002 Aug;17(2):201-10. [Content Brief]
[5]. Wu H, et al. Structural characterization of a dimerization interface in the CD28 transmembrane domain. Structure. 2022 Jun 2;30(6):803-812.e5. [Content Brief]
[6]. Kong KF, et al. A motif in the V3 domain of the kinase PKC-θ determines its localization in the immunological synapse and functions in T cells via association with CD28. Nat Immunol. 2011 Oct 2;12(11):1105-12. [Content Brief]
[7]. Blotta MH, et al. Cross-linking of the CD40 ligand on human CD4+ T lymphocytes generates a costimulatory signal that up-regulates IL-4 synthesis. J Immunol. 1996 May 1;156(9):3133-40. [Content Brief]
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