PDLIM1 Antibody (YA1565)(PBS only)

MedchemExpress Validation
Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), C2C12 (lane 2, 15 μg), and C6 (lane 3, 15 μg) using PDLIM1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:30000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

MedChemExpress Validation
Immunocytochemistry analysis of 293T cells labeling PDLIM1 Antibody (HY-P81820) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PDLIM1 Antibody (HY-P81820) dilution in BSA for Immunol Staining at 4 ℃overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

MedChemExpress Validation
Immunocytochemistry analysis of 293T cells labeling PDLIM1 Antibody (HY-P81820) at 1/200dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PDLIM1 Antibody (HY-P81820) dilution in BSA for Immunol Staining at 4 ℃overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).