PDE4B Antibody (YA2932)

Cat. No.: HY-P83187: Ficha de datos COA
SDS
User Guide for Antibodies
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PDE4B Antibody (YA2932) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PDE4B.

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Tamaño	Stock	Cantidad
20 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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PDE4B Antibody (YA2932) Recomendaciones destacadas:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Descripciòn

Descripciòn

PDE4B Antibody (YA2932) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PDE4B.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Peso molecular

Predicted band size: 83 kDa;

Observed band size: 95 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q07343

Gene ID

5142 [NCBI]

Immunogen

A synthesized peptide derived from human PDE4B aa500-736.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Pureza	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

ALL WB FC

Western blot analysis of extracts from A549(lane 2(20ug) and A549(lane 3(40ug) using PDE4B Antibody (HY-P83187) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Flow cytometric analysis of 1X10^6 A549 cells labeling PDE4B Antibody (YA2932) (HY-P82032, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

Function：Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (PubMed:15260978). May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents

Subcellular Localization：Cytoplasm; Cell membrane

Expression：
Tissue_specificity:Expressed in brain, heart, lung and skeletal muscle (PubMed:17519386) . Expressed in white blood cells (PubMed:8392015) ; Brain-specific isoform

Isoforms & Post-Translational Modification：Q07343 has 4 isomers: Q07343-1: 83343 Da (predicted); Q07343-2: 64352 Da (predicted); Q07343-3: 82096 Da (predicted); Q07343-4: 57709 Da (predicted).

Subunit：Interacts with DISC1

RRID

AB_3105302

Database

Entrez Gene: 5142 Human ; 24626 Rat

SwissProt: Q07343 Human ; P14646 Rat ;

OMIM: 600127 Human

Research Field

Signal Transduction

Synonyms

DPDE4; PDE 4B; PDE IVB; PDE32; Pde4b; PDE4B5; PDEIVB; Phosphodiesterase 4B

Documentación

Select Batch:

Ficha de datos (232 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

PDE4B Antibody (YA2932) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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PDE4B Antibody (YA2932)

PDE4B Antibody (YA2932) Recomendaciones destacadas:

Product Detail

Verification Image

Background

Descripciòn

PDE4B Antibody (YA2932) Related Classifications

Productos vistos recientemente:

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