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PDE4B Antibody (YA2932)

(Synonyms: DPDE4; PDE 4B; PDE IVB; PDE32; Pde4b; PDE4B5; PDEIVB; Phosphodiesterase 4B)
Customer Review

Based on 1 Customer Validation

PDE4B Antibody (YA2932) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PDE4B.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PDE4B Antibody (YA2932)
  • Experimental Validation Results for PDE4B Antibody (YA2932)
  • PDE4B Antibody (YA2932) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

PDE4B Antibody (YA2932) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PDE4B.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 95 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 83 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human PDE4B aa500-736.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105302

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PDE4B Antibody (YA2932)
    Western blot analysis of extracts from A549(lane 2(20ug) and A549(lane 3(40ug) using PDE4B Antibody (HY-P83187) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PDE4B Antibody (YA2932)
    Flow cytometric analysis of 1X10^6 A549 cells labeling PDE4B Antibody (YA2932) (HY-P82032, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    PDE4B hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents[1].

  • Subcellular Localization

    Cytoplasm; Cell membrane

  • Expression


    Tissue_specificity:Expressed in brain, heart, lung and skeletal muscle (PubMed:17519386) . Expressed in white blood cells (PubMed:8392015) ; Brain-specific isoform

  • Isoforms & Post-Translational Modification

    Q07343 has 4 isomers: Q07343-1: 83343 Da (predicted); Q07343-2: 64352 Da (predicted); Q07343-3: 82096 Da (predicted); Q07343-4: 57709 Da (predicted).

  • Subunit

    Interacts with DISC1

  • SwissProt ID

    Q07343

  • Gene ID
    5142 [NCBI]
  • Synonyms

    DPDE4; PDE 4B; PDE IVB; PDE32; Pde4b; PDE4B5; PDEIVB; Phosphodiesterase 4B

  • Research Field

    Signal Transduction

PDE4B Antibody (YA2932) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20ug) and A549(lane 3(40ug) using PDE4B Antibody (HY-P83187) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 A549 cells labeling PDE4B Antibody (YA2932) (HY-P82032, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • PDE4B Antibody (YA2932) Product Image
    1/16
  • Experimental Validation Results for PDE4B Antibody (YA2932)
  • Experimental Validation Results for PDE4B Antibody (YA2932)
  • PDE4B Antibody (YA2932) Product Image
  • Experimental Validation Results for PDE4B Antibody (YA2932)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20ug) and A549(lane 3(40ug) using PDE4B Antibody (HY-P83187) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PDE4B Antibody (YA2932)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 A549 cells labeling PDE4B Antibody (YA2932) (HY-P82032, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for PDE4B Antibody (YA2932)
  • Experimental Validation Results for PDE4B Antibody (YA2932)
  • PDE4B Antibody (YA2932) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549(lane 2(20ug) and A549(lane 3(40ug) using PDE4B Antibody (HY-P83187) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PDE4B Antibody (YA2932) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 A549 cells labeling PDE4B Antibody (YA2932) (HY-P82032, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • PDE4B Antibody (YA2932) Product Image WB
  • PDE4B Antibody (YA2932) Product Image FC