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  5. Wnt5a Antibody (YA5103)

Wnt5a Antibody (YA5103) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Wnt5a.

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容量 在庫状況
50 μL   お問い合わせ  
100 μL   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • 説明

製品説明

Wnt5a Antibody (YA5103) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Wnt5a.
Host

Rabbit
Clonality

Monoclonal,Recombinant
分子量
Predicted band size: 42 kDa;
Observed band size: 45 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human Wnt5a. The exact sequence is proprietary to MCE.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000-2000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-200
FC
FC: Flow Cytometry
1:50-100
Sensitivity Endogenous 純度 affinity purified by Protein A
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
輸送条件

Shipping with blue ice.
Background
Function：Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (By similarity). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor (PubMed:15735754). Mediates motility of melanoma cells (PubMed:17426020). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes (By similarity)
Subcellular Localization：Secreted, extracellular space, extracellular matrix; Secreted
Expression：
Tissue_specificity:This gene is expressed at elevated levels in differentiated thyroid carcinomas compared to normal thyroid tissue and undifferentiated thyroid tumors with low or undetectable expression levels. The gene is expressed in thyroid cells but not detected in stromal cells (protein level) (PubMed: 15735754) . It has also been detected in the hearts and lungs of newborns (PubMed: 8288227) .
Isoforms & Post-Translational Modification：P41221 has 2 isomers: P41221-1: 42339 Da (predicted); P41221-2: 40887 Da (predicted).
Glycosylation is necessary for secretion but not for activity;Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition;Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT5A
Subunit：Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity (PubMed:26902720). The complex with AFM may represent the physiological form in body fluids (PubMed:26902720). Homooligomer; disulfide-linked, leading to inactivation (in vitro). Interacts with PORCN. Interacts with WLS (By similarity). Interacts with glypican GCP3 (PubMed:14610063). Interacts with PKD1 (via extracellular domain) (PubMed:27214281). Interacts with TMEM67 (PubMed:34731008)
Synonyms
WNT5A; Protein Wnt-5a
ドキュメンテーション
User Guide for Antibodies (1077 KB)

Wnt5a Antibody (YA5103) 関連分類

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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製品名:
Wnt5a Antibody (YA5103)
製品番号:
HY-P85411
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