TSG-6 Antibody (YA9228)
(Synonyms: TSG6, TNFAIP6, Tumor necrosis factor-inducible gene 6 protein, Hyaluronate-binding protein, TNF-stimulated gene 6 protein, Tumor necrosis factor alpha-induced protein 6, TSG-6, TNF alpha-induced protein 6)
Based on 1 Customer Validation
TSG-6 Antibody (YA9228) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to TSG-6.
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Host:
Mouse
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Isotype:
IgG2b
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Application:
WB, ICC/IF, IF-Tissue, IHC-P, IP, ELISA
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Reactivity :
human
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Formulation:
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IP
IP: Immunoprecipitation
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:50-500 | 1:50-500 | 1:100-500 | 1-2μg per 100-500μg Total protein | 1:50-3000 |
Product Details
TSG-6 Antibody (YA9228) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to TSG-6.
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Host Mouse
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Species Reactivityhuman
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Calculated Molecular Weight Predicted band size: 35 kDa
OMIM: 600410
A synthesized peptide derived from human TSG-6.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG2b
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
TSG-6 is a Major regulator of extracellular matrix organization during tissue remodeling. Catalyzes the transfer of a heavy chain (HC) from inter-alpha-inhibitor (I-alpha-I) complex to hyaluronan. Cleaves the ester bond between the C-terminus of the HC and GalNAc residue of the chondroitin sulfate chain in I-alpha-I complex followed by transesterification of the HC to hyaluronan. In the process, potentiates the antiprotease function of I-alpha-I complex through release of free bikunin. Acts as a catalyst in the formation of hyaluronan-HC oligomers and hyaluronan-rich matrix surrounding the cumulus cell-oocyte complex, a necessary step for oocyte fertilization. Assembles hyaluronan in pericellular matrices that serve as platforms for receptor clustering and signaling. Enables binding of hyaluronan deposited on the surface of macrophages to LYVE1 on lymphatic endothelium and facilitates macrophage extravasation. Alters hyaluronan binding to functionally latent CD44 on vascular endothelium, switching CD44 into an active state that supports leukocyte rolling. Modulates the interaction of chemokines with extracellular matrix components and proteoglycans on endothelial cell surface, likely preventing chemokine gradient formation. In a negative feedback mechanism, may limit excessive neutrophil recruitment at inflammatory sites by antagonizing the association of CXCL8 with glycosaminoglycans on vascular endothelium. Has a role in osteogenesis and bone remodeling. Inhibits BMP2-dependent differentiation of mesenchymal stem cell to osteoblasts. Protects against bone erosion during inflammation by inhibiting TNFSF11/RANKL-dependent osteoclast activation[1][2][3][4][5][6][7][8][9][10][11].
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Subcellular Localization
Secreted
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Expression
Tissue_Specificity: Expressed in airway epithelium and submucosal gland (at protein level). Colocalizes with bikunin at the ciliary border. Present in bronchoalveolar lavage fluid (at protein level). Expressed in mesenchymal stem cells. Found in the synovial fluid of patients with rheumatoid arthritis.
Induction: Up-regulated in peripheral blood mononuclear cells, high endothelial venules, airway epithelium and submucosal gland in response to inflammatory cytokine TNF. Down-regulated upon differentiation of mesenchymal stem cells to osteoblasts. -
Isoforms & Post-Translational Modification
TSG-6 has an amino acid length of 277, molecular weight is 31203 Da.
N-glycosylated -
Subunit
Interacts (via Link domain) with inter-alpha-inhibitor (I-alpha-I) component bikunin.
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SwissProt ID
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Synonyms
TSG6, TNFAIP6, Tumor necrosis factor-inducible gene 6 protein, Hyaluronate-binding protein, TNF-stimulated gene 6 protein, Tumor necrosis factor alpha-induced protein 6, TSG-6, TNF alpha-induced protein 6
Documentation
References
[1]. Mahoney DJ, et al. Characterization of the interaction between tumor necrosis factor-stimulated gene-6 and heparin: implications for the inhibition of plasmin in extracellular matrix microenvironments. J Biol Chem. 2005 Jul 22;280(29):27044-55. [Content Brief]
[2]. Kuznetsova SA, et al. TSG-6 binds via its CUB_C domain to the cell-binding domain of fibronectin and increases fibronectin matrix assembly. Matrix Biol. 2008 Apr;27(3):201-10. [Content Brief]
[3]. Lawrance W, et al. Binding of Hyaluronan to the Native Lymphatic Vessel Endothelial Receptor LYVE-1 Is Critically Dependent on Receptor Clustering and Hyaluronan Organization. J Biol Chem. 2016 Apr 8;291(15):8014-30. [Content Brief]
[4]. Forteza R, et al. TSG-6 potentiates the antitissue kallikrein activity of inter-alpha-inhibitor through bikunin release. Am J Respir Cell Mol Biol. 2007 Jan;36(1):20-31. [Content Brief]
[5]. Sanggaard KW, et al. The TSG-6/HC2-mediated transfer is a dynamic process shuffling heavy chains between glycosaminoglycans. J Biol Chem. 2010 Jul 16;285(29):21988-93. [Content Brief]
[6]. Briggs DC, et al. Metal Ion-dependent Heavy Chain Transfer Activity of TSG-6 Mediates Assembly of the Cumulus-Oocyte Matrix. J Biol Chem. 2015 Nov 27;290(48):28708-23. [Content Brief]
[7]. Lesley J, et al. TSG-6 modulates the interaction between hyaluronan and cell surface CD44. J Biol Chem. 2004 Jun 11;279(24):25745-54. [Content Brief]
[8]. Dyer DP, et al. The Anti-inflammatory Protein TSG-6 Regulates Chemokine Function by Inhibiting Chemokine/Glycosaminoglycan Interactions. J Biol Chem. 2016 Jun 10;291(24):12627-12640. [Content Brief]
[9]. Dyer DP, et al. TSG-6 inhibits neutrophil migration via direct interaction with the chemokine CXCL8. J Immunol. 2014 Mar 1;192(5):2177-85. [Content Brief]
[10]. Tsukahara S, et al. Tumour necrosis factor alpha-stimulated gene-6 inhibits osteoblastic differentiation of human mesenchymal stem cells induced by osteogenic differentiation medium and BMP-2. Biochem J. 2006 Sep 15;398(3):595-603. [Content Brief]
[11]. Mahoney DJ, et al. TSG-6 regulates bone remodeling through inhibition of osteoblastogenesis and osteoclast activation. J Biol Chem. 2008 Sep 19;283(38):25952-62. [Content Brief]
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