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ICAM1/CD54 Antibody (YA352)

(Synonyms: ICAM1; Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD antigen CD54)
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Based on 1 Customer Validation

ICAM1/CD54 Antibody (YA352) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ICAM1/CD54.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ICAM1/CD54 Antibody (YA352)
  • Experimental Validation Results for ICAM1/CD54 Antibody (YA352)
  • ICAM1/CD54 Antibody (YA352) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
Predicted band size Info
 
Observed band size Info
 
Dilution Ratio 58 kDa 89/92 kDa

Product Details

Description

ICAM1/CD54 Antibody (YA352) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ICAM1/CD54.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 89/92 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 58 kDa
Species Reactivity Database

Entrez Gene: 3383 Human

SwissProt: P05362 Human

Immunogen

Synthetic peptide corresponding to Human ICAM1.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102675

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ICAM1/CD54 Antibody (YA352)
    Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), HeLa (lane 4), HepG2 (lane 5) and A549 (lane 6) using ICAM1/CD54 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for ICAM1/CD54 Antibody (YA352)
    Western blot analysis of extracts from K562(lane 2(20μg) and K562(lane 3(40μg), Hela(lane 4(20μg) ) and Hela(lane 5(40μg) ) using ICAM1 (HY-P80716) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80716, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation; (Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins; (Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins; (Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell

  • Subcellular Localization

    Membrane; Single-pass type I membrane protein

  • Expression


    Induction:Up-regulated by coagulation factor Xa (F10) in atrial tissues (PubMed:24041930) . Up-regulated in atrial tissues by rapid pacing resembling atrial fibrillation (PubMed:24041930)

  • Subunit

    Homodimer (Probable). Interacts with MUC1 and promotes cell aggregation in epithelial cells. Interacts with ARHGEF26/SGEF. Interacts (on T cell side) with CD81, CD247 and CD9 at immunological synapses between antigen-presenting cells and T cells

  • SwissProt ID

    P05362

  • Gene ID
    3383 [NCBI]
  • Synonyms

    ICAM1; Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD antigen CD54

  • Research Field

    Immunology

ICAM1/CD54 Antibody (YA352) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), HeLa (lane 4), HepG2 (lane 5) and A549 (lane 6) using ICAM1/CD54 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20μg) and K562(lane 3(40μg), Hela(lane 4(20μg) ) and Hela(lane 5(40μg) ) using ICAM1 (HY-P80716) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80716, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • ICAM1/CD54 Antibody (YA352) Product Image
    1/16
  • Experimental Validation Results for ICAM1/CD54 Antibody (YA352)
  • Experimental Validation Results for ICAM1/CD54 Antibody (YA352)
  • ICAM1/CD54 Antibody (YA352) Product Image
  • Experimental Validation Results for ICAM1/CD54 Antibody (YA352)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), HeLa (lane 4), HepG2 (lane 5) and A549 (lane 6) using ICAM1/CD54 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for ICAM1/CD54 Antibody (YA352)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20μg) and K562(lane 3(40μg), Hela(lane 4(20μg) ) and Hela(lane 5(40μg) ) using ICAM1 (HY-P80716) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80716, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ICAM1/CD54 Antibody (YA352)
  • Experimental Validation Results for ICAM1/CD54 Antibody (YA352)
  • ICAM1/CD54 Antibody (YA352) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), HeLa (lane 4), HepG2 (lane 5) and A549 (lane 6) using ICAM1/CD54 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • ICAM1/CD54 Antibody (YA352) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from K562(lane 2(20μg) and K562(lane 3(40μg), Hela(lane 4(20μg) ) and Hela(lane 5(40μg) ) using ICAM1 (HY-P80716) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80716, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • ICAM1/CD54 Antibody (YA352) Product Image WB
  • ICAM1/CD54 Antibody (YA352) Product Image WB