BAP1 Antibody (YA2733)

Cat. No.: HY-P82988: Data Sheet COA
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BAP1 Antibody (YA2733) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BAP1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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BAP1 Antibody (YA2733) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

BAP1 Antibody (YA2733) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BAP1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 80 kDa;

Observed band size: 95 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q92560

Gene ID

8314 [NCBI]

Immunogen

Recombinant protein of human BAP1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1 (PubMed:12485996, PubMed:18757409, PubMed:20436459, PubMed:25451922, PubMed:35051358). Catalytic component of the polycomb repressive deubiquitinase (PR-DUB) complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-120' (H2AK119ub1) (PubMed:20436459, PubMed:25451922, PubMed:30664650, PubMed:35051358). Does not deubiquitinate monoubiquitinated histone H2B (PubMed:20436459, PubMed:30664650). The PR-DUB complex is an epigenetic regulator of gene expression and acts as a transcriptional coactivator, affecting genes involved in development, cell communication, signaling, cell proliferation and cell viability (PubMed:20805357, PubMed:30664650, PubMed:36180891). Antagonizes PRC1 mediated H2AK119ub1 monoubiquitination (PubMed:30664650). As part of the PR-DUB complex, associates with chromatin enriched in histone marks H3K4me1, H3K4me3, and H3K27Ac, but not in H3K27me3 (PubMed:36180891). Recruited to specific gene-regulatory regions by YY1 (PubMed:20805357). Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains (PubMed:19188440, PubMed:19815555). Deubiquitination of HCFC1 does not lead to increase stability of HCFC1 (PubMed:19188440, PubMed:19815555). Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination (PubMed:19117993). It however does not mediate deubiquitination of BRCA1 and BARD1 (PubMed:19117993). Able to mediate autodeubiquitination via intramolecular interactions to counteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration (PubMed:24703950). Negatively regulates epithelial-mesenchymal transition (EMT) of trophoblast stem cells during placental development by regulating genes involved in epithelial cell integrity, cell adhesion and cytoskeletal organization (PubMed:34170818)

Subcellular Localization：Cytoplasm; Nucleus; Chromosome

Expression：
Tissue_specificity:Highly expressed in testis, placenta and ovary (PubMed:9528852) . Expressed in breast (PubMed:9528852) . levels in the placenta increase over the course of pregnancy (PubMed:34170818)

Subunit：Core component of the polycomb repressive deubiquitinase (PR-DUB) complex, at least composed of BAP1, one of ASXL1, ASXL2 or (probably) ASXL3, and one of MBD5 or MBD6 (PubMed:20436459, PubMed:24634419, PubMed:30664650, PubMed:36180891). The PR-DUB core associates with a number of accessory proteins, including FOXK1, FOXK2, KDM1B, HCFC1, YY1 and OGT; KDM1B specifically associates with ASXL2 PR-DUB complexes (Probable) (PubMed:20805357, PubMed:30664650). The BAP1 deubiquitinase activity is not required for PR-DUB assembly (PubMed:20805357). Homodimerizes (via coiled-coil hinge-region between the UCH and ULD domains) to mediate assembly of 2 copies of the BAP1-ASXL heterodimer into a bisymmetric tetramer; dimerization enhances association with nucleosomes (PubMed:30258054, PubMed:35446349). The PR-DUB complex associates with nucleosomes to mediate deubiquitination of 'lys-120' of histone H2AK118ub1 substrates; the association requires the positively charged C-terminal tail of BAP1 (PubMed:30258054, PubMed:36991118, PubMed:37556531). Interacts (via ULD domain) with ASXL1 (via DEUBAD domain); the interaction is direct and forms a ubiquitin binding cleft (PubMed:36180891, PubMed:36991118, PubMed:37556531). The interaction with ASXL1 stabilizes BAP1 but is not required for nucleosome binding (PubMed:36180891). Associates (via C-terminus) with nucleosome and chromatosome complexes through direct interaction with DNA and the histone3/4 dimer; this association displaces the histone-2A C-terminal tail, extending and orienting the H2AK118ub1 substrate towards the BAP1 deubiquitinase active site (PubMed:36991118, PubMed:37556531). Also interacts (via arginine finger) directly with the histone H2A-H2B acidic patch; this interaction is not critical for nucleosome-chromatosome association but may play a role in orienting the H2AK118ub1 substrate towards the PR-DUB complex active site (PubMed:36991118). Interacts with BRCA1 (via the RING finger) (PubMed:19117993, PubMed:9528852). Interacts (via HBM-like motif) with HCFC1 (PubMed:19188440, PubMed:19815555, PubMed:20805357). Interacts (via a C-terminal region overlapping the ULD domain) with YY1; the interaction is direct and requires the interaction with HCFC1 (PubMed:20805357). Interacts (when phosphorylated at Thr-493) with FOXK1 (PubMed:25451922). Interacts (when phosphorylated at Thr-493) with FOXK2; leading to recruitment of the PR-DUB complex and repression of FOXK2 target genes (PubMed:24748658, PubMed:25451922). Interacts (via non-classical PY-NLS) with TNPO1/transportin-1 (via HEAT repeats 8-12); the interaction is direct, mediates BAP1 nuclear localization and disrupts BAP1 homodimerization (PubMed:35446349). Interacts (via C-terminus) with KPNA1/importin alpha5 and KPNA2/importin alpha1; these interactions can contribute to BAP1 nuclear localization but are less important than the interaction with TNPO1/transportin-1 (PubMed:35446349). The interaction with TNPO1/transportin-1 disrupts homodimerization and blocks ubiquitination by UBE2O (PubMed:35446349)

Database

Entrez Gene: 8314 Human

SwissProt: Q92560 Human

OMIM: 156240 Human

Research Field

Cell Biology

Synonyms

UCHL2; hucep-6; HUCEP-13

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/lox-imvi-cell.html [Content Brief]

BAP1 Antibody (YA2733) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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BAP1 Antibody (YA2733)

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