FOLR1 Antibody (YA9643)
(Synonyms: FOLR1; FOLR; Folate receptor alpha; FR-alpha; Adult folate-binding protein; FBP; Folate receptor 1; Folate receptor; adult; KB cells FBP; Ovarian tumor-associated antigen MOv18)FOLR1 Antibody (YA9643) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Folate Binding Protein.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IF-Tissue
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Reactivity :
Human
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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|---|---|---|---|
| Dilution Ratio | 1:5,000 | 1:5,000 | 1:1,000 |
Product Details
FOLR1 Antibody (YA9643) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Folate Binding Protein.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 40 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 30 kDa
Recombinant protein within human FOLR1 aa 25-257.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
FOLR1 binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. Required for normal embryonic development and normal cell proliferation (By similarity)[1][2][3][4][5][6].
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Subcellular Localization
Cell membrane; Apical cell membrane; Basolateral cell membrane; Secreted; Cytoplasmic vesicle; Cytoplasmic vesicle, clathrin-coated vesicle; Endosome
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Expression
Tissue_Specificity: Primarily expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum. Detected in placenta and thymus epithelium. -
Isoforms & Post-Translational Modification
FOLR1 has an amino acid length of 257, molecular weight is 29819 Da.
The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease -
SwissProt ID
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Synonyms
FOLR1; FOLR; Folate receptor alpha; FR-alpha; Adult folate-binding protein; FBP; Folate receptor 1; Folate receptor; adult; KB cells FBP; Ovarian tumor-associated antigen MOv18
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Research Field
Tags & Cell Markers
Documentation
References
[1]. Zhao R, et al. A role for the proton-coupled folate transporter (PCFT-SLC46A1) in folate receptor-mediated endocytosis. J Biol Chem. 2009 Feb 13;284(7):4267-74. [Content Brief]
[2]. Chen C, et al. Structural basis for molecular recognition of folic acid by folate receptors. Nature. 2013 Aug 22;500(7463):486-9. [Content Brief]
[3]. Wibowo AS, et al. Structures of human folate receptors reveal biological trafficking states and diversity in folate and antifolate recognition. Proc Natl Acad Sci U S A. 2013 Sep 17;110(38):15180-8. [Content Brief]
[4]. Lacey SW, et al. Complementary DNA for the folate binding protein correctly predicts anchoring to the membrane by glycosyl-phosphatidylinositol. J Clin Invest. 1989 Aug;84(2):715-20. [Content Brief]
[5]. Orr RB, et al. UMSCC38 cells amplified at 11q13 for the folate receptor synthesize a mutant nonfunctional folate receptor. Cancer Res. 1994 Jul 15;54(14):3905-11. [Content Brief]
[6]. Rijnboutt S, et al. Endocytosis of GPI-linked membrane folate receptor-alpha. J Cell Biol. 1996 Jan;132(1-2):35-47. [Content Brief]
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