PHF1 Antibody
(Synonyms: PCL1, PHF1, PHD finger protein 1, Protein PHF1, hPHF1, Polycomb-like protein 1, hPCl1)PHF1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to PHF1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Bovine
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
PHF1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to PHF1.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Bovine
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Observed Molecular WeightObserved band size: 62 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 62 kDa
Synthetic peptide corresponding to the center region of human PHF1.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Soulution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PHF1 is a Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci. According to another report, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2. Regulates p53/TP53 stability and prolonges its turnover: may act by specifically binding to a methylated from of p53/TP53[1][2][3].
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Subcellular Localization
Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
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Expression
Tissue_Specificity: Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney. -
Subunit
Interacts with CHMP1 (By similarity).
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SwissProt ID
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Synonyms
PCL1, PHF1, PHD finger protein 1, Protein PHF1, hPHF1, Polycomb-like protein 1, hPCl1
Documentation
[1]. Sarma K, et al. Ezh2 requires PHF1 to efficiently catalyze H3 lysine 27 trimethylation in vivo. Mol Cell Biol. 2008 Apr;28(8):2718-31. [Content Brief]
[2]. Cai L, et al. An H3K36 methylation-engaging Tudor motif of polycomb-like proteins mediates PRC2 complex targeting. Mol Cell. 2013 Feb 7;49(3):571-82. [Content Brief]
[3]. Musselman CA, et al. Molecular basis for H3K36me3 recognition by the Tudor domain of PHF1. Nat Struct Mol Biol. 2012 Dec;19(12):1266-72. [Content Brief]
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