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Histone H4 (acetyl K5) Antibody (YA362)

(Synonyms: H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4, H4K5ac)
2 Cited Publications
Customer Review

Based on 2 publication(s) in Google Scholar

Histone H4 (acetyl K5) Antibody (YA362) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Histone H4 (acetyl K5).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, IP, ChIP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
  • Histone H4 (acetyl K5) Antibody (YA362) Product Image
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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
ChIP Info
ChIP: Chromatin Immunoprecipitation
Dilution Ratio 1:1000-1:5000 1:50-1:400 1:50-1:1000 Use at an assay dependent concentration Use 0.5~2 μg for 25 μg of chromatin.

Product Details

Description

Histone H4 (acetyl K5) Antibody (YA362) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Histone H4 (acetyl K5).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 11 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 11 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Histone H4.AA range:1-50.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Acetylated

Isotype

IgG

RRID

AB_3102851

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
    Western blot analysis of extracts from Hela (lane2(20μg), C6(lane3(20μg), NIH/3T3(lane4(20μg) and HepG2(lane5(20μg) using Histone H4 (acetyl K5) Antibody (HY-P80182). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
    Immunohistochemical analysis of paraffin-embedded mouse testicle tissue using Histone H4 (acetyl K5) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
    Immunohistochemical analysis of paraffin-embedded mouse testicle tissue using Histone H4 (acetyl K5) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
    Immunocytochemistry analysis of NIH/3T3 cells labeling Histone H4 (acetyl K5) with Histone H4 (acetyl K5) Antibody (YA362) (HY-P81082) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Histone H4 (acetyl K5) Antibody (YA362) ((HY-P81082) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
    Immunocytochemistry analysis of Hela cells labeling Histone H4 (acetyl K5) with Histone H4 (acetyl K5) Antibody (YA362) (HY-P81082) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Histone H4 (acetyl K5) Antibody (YA362) ((HY-P81082) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Histone H4 core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling

  • Subcellular Localization

    Nucleus; Chromosome

  • Subunit

    The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers.

  • SwissProt ID

    P62805

  • Gene ID
    121504 [NCBI]
  • Synonyms

    H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4, H4K5ac

  • Research Field

    Epigenetics and Nuclear Signaling

Histone H4 (acetyl K5) Antibody (YA362) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), C6(lane3(20μg), NIH/3T3(lane4(20μg) and HepG2(lane5(20μg) using Histone H4 (acetyl K5) Antibody (HY-P80182). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse testicle tissue using Histone H4 (acetyl K5) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse testicle tissue using Histone H4 (acetyl K5) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of NIH/3T3 cells labeling Histone H4 (acetyl K5) with Histone H4 (acetyl K5) Antibody (YA362) (HY-P81082) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Histone H4 (acetyl K5) Antibody (YA362) ((HY-P81082) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Histone H4 (acetyl K5) with Histone H4 (acetyl K5) Antibody (YA362) (HY-P81082) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Histone H4 (acetyl K5) Antibody (YA362) ((HY-P81082) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Histone H4 (acetyl K5) Antibody (YA362) Product Image
    1/16
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
  • Histone H4 (acetyl K5) Antibody (YA362) Product Image
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), C6(lane3(20μg), NIH/3T3(lane4(20μg) and HepG2(lane5(20μg) using Histone H4 (acetyl K5) Antibody (HY-P80182). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse testicle tissue using Histone H4 (acetyl K5) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse testicle tissue using Histone H4 (acetyl K5) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of NIH/3T3 cells labeling Histone H4 (acetyl K5) with Histone H4 (acetyl K5) Antibody (YA362) (HY-P81082) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Histone H4 (acetyl K5) Antibody (YA362) ((HY-P81082) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Histone H4 (acetyl K5) with Histone H4 (acetyl K5) Antibody (YA362) (HY-P81082) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Histone H4 (acetyl K5) Antibody (YA362) ((HY-P81082) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
  • Experimental Validation Results for Histone H4 (acetyl K5) Antibody (YA362)
  • Histone H4 (acetyl K5) Antibody (YA362) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2(20μg), C6(lane3(20μg), NIH/3T3(lane4(20μg) and HepG2(lane5(20μg) using Histone H4 (acetyl K5) Antibody (HY-P80182). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Histone H4 (acetyl K5) Antibody (YA362) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse testicle tissue using Histone H4 (acetyl K5) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Histone H4 (acetyl K5) Antibody (YA362) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse testicle tissue using Histone H4 (acetyl K5) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Histone H4 (acetyl K5) Antibody (YA362) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of NIH/3T3 cells labeling Histone H4 (acetyl K5) with Histone H4 (acetyl K5) Antibody (YA362) (HY-P81082) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Histone H4 (acetyl K5) Antibody (YA362) ((HY-P81082) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Histone H4 (acetyl K5) Antibody (YA362) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Histone H4 (acetyl K5) with Histone H4 (acetyl K5) Antibody (YA362) (HY-P81082) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Histone H4 (acetyl K5) Antibody (YA362) ((HY-P81082) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Histone H4 (acetyl K5) Antibody (YA362) Product Image WB
  • Histone H4 (acetyl K5) Antibody (YA362) Product Image IHC-P
  • Histone H4 (acetyl K5) Antibody (YA362) Product Image IHC-P
  • Histone H4 (acetyl K5) Antibody (YA362) Product Image ICC
  • Histone H4 (acetyl K5) Antibody (YA362) Product Image ICC