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Cyclin T1 Antibody (YA2600)(PBS only)

(Synonyms: CCNT; CYCT1; HIVE1)

Cyclin T1 Antibody (YA2600) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cyclin T1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cyclin T1 Antibody (YA2600)(PBS only)
  • Cyclin T1 Antibody (YA2600)(PBS only) Product Image
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200 1:20

Product Details

Description

Cyclin T1 Antibody (YA2600) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cyclin T1.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 81 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 81 kDa
Immunogen

Recombinant protein of human Cyclin T1

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cyclin T1 Antibody (YA2600)(PBS only)
    Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), HeLa (lane 2, 15 μg), HepG2 (lane 3, 15 μg), and RAW264.7 (lane 4, 15 μg) using Cyclin T1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Cyclin T1 is a Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). Required to activate the protein kinase activity of CDK9: acts by mediating formation of liquid-liquid phase separation (LLPS) that enhances binding of P-TEFb to the CTD of RNA Pol II; (Microbial infection) In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes[1][2][3][4][5].

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:Ubiquitously expressed

  • Isoforms & Post-Translational Modification

    O60563 has 2 isomers: O60563-1: 80685 Da (predicted); O60563-2: 21206 Da (predicted).
    ADP-ribosylation on serine residues by PARP1 in response to DNA damage disrupts the phase separation activity of CCNT1, thereby preventing activation of CDK9

  • Subunit

    Cyclin-T1 is the predominant cyclin that associates with CDK9 to form a heterodimer called P-TEFb (PubMed:30134174, PubMed:35393539, PubMed:9499409). P-TEFb forms a complex with AFF4/AF5Q31 (PubMed:12065898). Component of a complex which is at least composed of HTATSF1/Tat-SF1, P-TEFb complex, RNA pol II, SUPT5H, and NCL/nucleolin (PubMed:10393184). Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs (PubMed:17643375). Interacts (via central region) with ZMYND8 (via N-terminus); the interaction is direct and the association appears to occur between homodimeric ZMYND8 and the activated form of the P-TEFb complex (PubMed:30134174). Interacts with BRD4, targets chromatin binding (PubMed:16109376, PubMed:16109377, PubMed:24360279). Interacts with JMJD6 (PubMed:24360279). Interacts with MDFIC (PubMed:12944466). Interacts with HSF1 (PubMed:27189267). Interacts with HTATSF1 (PubMed:10913173). Interacts with TBX21 (PubMed:27292648)

  • SwissProt ID

    O60563

  • Gene ID
    904 [NCBI]
  • Synonyms

    CCNT; CYCT1; HIVE1

  • Research Field

    Microbiology

Cyclin T1 Antibody (YA2600)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), HeLa (lane 2, 15 μg), HepG2 (lane 3, 15 μg), and RAW264.7 (lane 4, 15 μg) using Cyclin T1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Cyclin T1 Antibody (YA2600)(PBS only) Product Image
    1/16
  • Experimental Validation Results for Cyclin T1 Antibody (YA2600)(PBS only)
  • Cyclin T1 Antibody (YA2600)(PBS only) Product Image
  • Experimental Validation Results for Cyclin T1 Antibody (YA2600)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), HeLa (lane 2, 15 μg), HepG2 (lane 3, 15 μg), and RAW264.7 (lane 4, 15 μg) using Cyclin T1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Cyclin T1 Antibody (YA2600)(PBS only)
  • Cyclin T1 Antibody (YA2600)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), HeLa (lane 2, 15 μg), HepG2 (lane 3, 15 μg), and RAW264.7 (lane 4, 15 μg) using Cyclin T1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Cyclin T1 Antibody (YA2600)(PBS only) Product Image WB