PRPF4 Antibody (YA1757)(PBS only)

Cat. No.: HY-P82012A: Data Sheet User Guide for Antibodies
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PRPF4 Antibody (YA1757) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRPF4.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PRPF4 Antibody (YA1757) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRPF4.

Host

Rabbit

Molecular Weight

Predicted band size: 58 kDa;

Observed band size: 58 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O43172

Gene ID

9128 [NCBI]

Immunogen

A synthetic peptide of human PRPF4

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:500-1:1000
FC FC: Flow Cytometry	1:20-1:50
IP IP: Immunoprecipitation	1:20-1:50

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex)

Subcellular Localization：Nucleus; Nucleus speckle

Isoforms & Post-Translational Modification：O43172 has 2 isomers: O43172-1: 58449 Da (predicted); O43172-2: 58321 Da (predicted).

Subunit：Component of the precatalytic spliceosome (spliceosome B complex) (PubMed:28781166). Component of the U4/U6-U5 tri-snRNP complex, a building block of the precatalytic spliceosome (spliceosome B complex) (PubMed:26912367, PubMed:28781166, PubMed:9257651, PubMed:9328476, PubMed:9404889). The U4/U6-U5 tri-snRNP complex is composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, plus LSM2, LSM3, LSM4, LSM5, LSM6, LSM7 and LSM8 (PubMed:16723661, PubMed:26912367). Interacts directly with PRPF18, PPIH and PRPF3 (PubMed:12875835, PubMed:25383878, PubMed:9000057, PubMed:9328476, PubMed:9404889). Part of a heteromeric complex containing PPIH, PRPF3 and PRPF4 that is stable in the absence of RNA (PubMed:9404889). Interacts with ERCC6 (PubMed:26030138)

Research Field

Cell Biology

Synonyms

PRP4; RP70; HPRP4; Prp4p; HPRP4P; SNRNP60

Documentation

User Guide for Antibodies (1077 KB)