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Integrin α5 Antibody (YA7426)

Customer Review

Based on 1 Customer Validation

Integrin α5 Antibody (YA7426) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Integrin α5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG,Kappa

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Integrin α5 Antibody (YA7426)
  • Experimental Validation Results for Integrin α5 Antibody (YA7426)
  • Experimental Validation Results for Integrin α5 Antibody (YA7426)
  • Integrin α5 Antibody (YA7426) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:100-1:500 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

Integrin α5 Antibody (YA7426) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Integrin α5.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 150,19 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 115 kDa
Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG,Kappa

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Integrin α5 Antibody (YA7426)
    Western blot analysis of extracts from A549 (lane 2(20μg), C6 (lane 3(20μg), Hela (lane 4(20μg) and NIH3T3 (lane 5(20μg) using Integrin α5 Antibody (HY-P87741) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Integrin α5 Antibody (YA7426)
    Immunocytochemistry analysis of A549 cells labeling Integrin α5 Antibody (HY-P87741) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin α5 Antibody (HY-P87741) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Integrin α5 Antibody (YA7426)
    Immunocytochemistry analysis of NIH/3T3 cells labeling Integrin α5 Antibody (HY-P87741) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin α5 Antibody (HY-P87741) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Integrin α5 is an Integrin alpha-5/beta-1 (ITGA5:ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. ITGA5:ITGB1 acts as a receptor for fibronectin (FN1) and mediates R-G-D-dependent cell adhesion to FN1. ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling. ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling |(Microbial infection) Integrin ITGA5:ITGB1 acts as a receptor for Human metapneumovirus|(Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human parvovirus B19|(Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions[1][2][3][4][5][6][7].

  • Subcellular Localization

    Cell membrane,Cell junction, focal adhesion

  • Expression


    Tissue_Specificity: Expressed in placenta (at protein level)

  • Isoforms & Post-Translational Modification

    P08648: 1049 amino acids, molecular weight 114536 Da.

  • Subunit

    Heterodimer of an alpha and a beta subunit

  • SwissProt ID

    P08648

Integrin α5 Antibody (YA7426) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 2(20μg), C6 (lane 3(20μg), Hela (lane 4(20μg) and NIH3T3 (lane 5(20μg) using Integrin α5 Antibody (HY-P87741) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Integrin α5 Antibody (HY-P87741) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin α5 Antibody (HY-P87741) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of NIH/3T3 cells labeling Integrin α5 Antibody (HY-P87741) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin α5 Antibody (HY-P87741) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Integrin α5 Antibody (YA7426) Product Image
    1/16
  • Experimental Validation Results for Integrin α5 Antibody (YA7426)
  • Experimental Validation Results for Integrin α5 Antibody (YA7426)
  • Experimental Validation Results for Integrin α5 Antibody (YA7426)
  • Integrin α5 Antibody (YA7426) Product Image
  • Experimental Validation Results for Integrin α5 Antibody (YA7426)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 2(20μg), C6 (lane 3(20μg), Hela (lane 4(20μg) and NIH3T3 (lane 5(20μg) using Integrin α5 Antibody (HY-P87741) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Integrin α5 Antibody (YA7426)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Integrin α5 Antibody (HY-P87741) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin α5 Antibody (HY-P87741) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Integrin α5 Antibody (YA7426)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of NIH/3T3 cells labeling Integrin α5 Antibody (HY-P87741) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin α5 Antibody (HY-P87741) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Integrin α5 Antibody (YA7426)
  • Experimental Validation Results for Integrin α5 Antibody (YA7426)
  • Experimental Validation Results for Integrin α5 Antibody (YA7426)
  • Integrin α5 Antibody (YA7426) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549 (lane 2(20μg), C6 (lane 3(20μg), Hela (lane 4(20μg) and NIH3T3 (lane 5(20μg) using Integrin α5 Antibody (HY-P87741) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Integrin α5 Antibody (YA7426) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling Integrin α5 Antibody (HY-P87741) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin α5 Antibody (HY-P87741) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Integrin α5 Antibody (YA7426) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of NIH/3T3 cells labeling Integrin α5 Antibody (HY-P87741) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Integrin α5 Antibody (HY-P87741) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Integrin α5 Antibody (YA7426) Product Image WB
  • Integrin α5 Antibody (YA7426) Product Image ICC
  • Integrin α5 Antibody (YA7426) Product Image ICC