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FABP4 Antibody (YA449)

2 Cited Publications
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Based on 2 publication(s) in Google Scholar

FABP4 Antibody (YA449) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FABP4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for FABP4 Antibody (YA449)
  • Experimental Validation Results for FABP4 Antibody (YA449)
  • Experimental Validation Results for FABP4 Antibody (YA449)
  • FABP4 Antibody (YA449) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
Dilution Ratio 1:500-1:2000 1:100-1:500 1:100

Product Details

Description

FABP4 Antibody (YA449) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FABP4.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 15 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 15 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human FABP4.AA range:60-100.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102084

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FABP4 Antibody (YA449)
    Western blot analysis of extracts from 3T3-L1 (lane 2(20μg) and 3T3-L1 (lane 3(40μg), HEK293(lane 4(20μg) and HEK293(lane 5(40μg) using FABP4 (HY-P80124) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FABP4 Antibody (YA449)
    Immunocytochemistry analysis of 3T3-L1 cells labeling FABP4 with FABP4 Antibody (HY-P80124)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FABP4 Antibody (HY-P80124) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for FABP4 Antibody (YA449)
    Immunocytochemistry analysis of 3T3-L1 cells labeling FABP4 with FABP4 Antibody (HY-P80124) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FABP4 Antibody (HY-P80124) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Subunit

    Monomer (PubMed:15357969, PubMed:17077479). Homodimer. Interacts with PPARG (By similarity)

  • SwissProt ID

    P15090

  • Gene ID
    2167 [NCBI]
  • Research Field

    Cardiovascular

FABP4 Antibody (YA449) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from 3T3-L1 (lane 2(20μg) and 3T3-L1 (lane 3(40μg), HEK293(lane 4(20μg) and HEK293(lane 5(40μg) using FABP4 (HY-P80124) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of 3T3-L1 cells labeling FABP4 with FABP4 Antibody (HY-P80124)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FABP4 Antibody (HY-P80124) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of 3T3-L1 cells labeling FABP4 with FABP4 Antibody (HY-P80124) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FABP4 Antibody (HY-P80124) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • FABP4 Antibody (YA449) Product Image
    1/16
  • Experimental Validation Results for FABP4 Antibody (YA449)
  • Experimental Validation Results for FABP4 Antibody (YA449)
  • Experimental Validation Results for FABP4 Antibody (YA449)
  • FABP4 Antibody (YA449) Product Image
  • Experimental Validation Results for FABP4 Antibody (YA449)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from 3T3-L1 (lane 2(20μg) and 3T3-L1 (lane 3(40μg), HEK293(lane 4(20μg) and HEK293(lane 5(40μg) using FABP4 (HY-P80124) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FABP4 Antibody (YA449)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of 3T3-L1 cells labeling FABP4 with FABP4 Antibody (HY-P80124)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FABP4 Antibody (HY-P80124) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for FABP4 Antibody (YA449)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of 3T3-L1 cells labeling FABP4 with FABP4 Antibody (HY-P80124) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FABP4 Antibody (HY-P80124) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for FABP4 Antibody (YA449)
  • Experimental Validation Results for FABP4 Antibody (YA449)
  • Experimental Validation Results for FABP4 Antibody (YA449)
  • FABP4 Antibody (YA449) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from 3T3-L1 (lane 2(20μg) and 3T3-L1 (lane 3(40μg), HEK293(lane 4(20μg) and HEK293(lane 5(40μg) using FABP4 (HY-P80124) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • FABP4 Antibody (YA449) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of 3T3-L1 cells labeling FABP4 with FABP4 Antibody (HY-P80124)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FABP4 Antibody (HY-P80124) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • FABP4 Antibody (YA449) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of 3T3-L1 cells labeling FABP4 with FABP4 Antibody (HY-P80124) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with FABP4 Antibody (HY-P80124) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • FABP4 Antibody (YA449) Product Image WB
  • FABP4 Antibody (YA449) Product Image ICC
  • FABP4 Antibody (YA449) Product Image ICC