Indoleamine 2,3-dioxygenase Antibody (YA3100)(PBS only)

Cat. No.: HY-P83355A: Data Sheet User Guide for Antibodies
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Indoleamine 2,3-dioxygenase Antibody (YA3100) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Indoleamine 2,3-dioxygenase.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Indoleamine 2,3-dioxygenase Antibody (YA3100) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Indoleamine 2,3-dioxygenase.

Host

Rabbit

Molecular Weight

Predicted band size: 45 kDa;

Observed band size: 45 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P14902

Gene ID

3620 [NCBI]

Immunogen

Recombinant protein of human Indoleamine 2,3-dioxygenase aa1-403.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed:17671174). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed:25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed:25691885). Acts as a suppressor of anti-tumor immunity (PubMed:14502282, PubMed:23103127, PubMed:25157255, PubMed:25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed:25691885). Protects the fetus from maternal immune rejection (PubMed:25691885)

Subcellular Localization：Cytoplasm, cytosol

Expression：
Tissue_specificity:Expressed in mature dendritic cells located in lymphoid organs (including lymph nodes, spleen, tonsils, Peyers's patches, the gut lamina propria, and the thymic medulla) , in some epithelial cells of the female genital tract, as well as in endothelial cells of term placenta and in lung parenchyma (PubMed:25691885) . Weakly or not expressed in most normal tissues, but mostly inducible in most tissues (PubMed:25691885) . Expressed in more than 50% of tumors, either by tumoral, stromal, or endothelial cells (expression in tumor is associated with a worse clinical outcome) (PubMed:18418598) . Not overexpressed in tumor-draining lymph nodes (PubMed:25691885, PubMed:26155395)

Induction:By IFNG/IFN-gamma in most cells (PubMed:1907934, PubMed:2109605) . Exogenous inflammatory stimuli induce the expression of IDO1 in antigen-presenting cells such as dendritic cells, macrophages and B-cells (PubMed:25157255) .

Subunit：Monomer

Research Field

Signal Transduction

Synonyms

3-dioxygenase; IDO 1; IDO; IDO1; INDO; indolamine 2; 3 dioxygenase; Indole 2 3 dioxygenase

Documentation

User Guide for Antibodies (1077 KB)