Annexin II Antibody (YA7408)
Based on 1 Customer Validation
Annexin II Antibody (YA7408) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Annexin II.
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Host:
Rabbit
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Isotype:
IgG,Kappa
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Application:
WB, IHC-P, ICC/IF, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:2000-1:10000 | 1:2000-1:10000 | 1:200-1:1000 | 1:5000-1:20000 |
Product Details
Annexin II Antibody (YA7408) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Annexin II.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 39 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 39 kDa
The exact sequence is proprietary to MCE.
Endogenous
Protein A affinity purified
Non-conjugated
Unmodified
IgG,Kappa
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from A549 (lane 1(20μg)) 、HeLa (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) 、RAW264.7 (lane 4(20μg)) and C6 (lane 5(20μg)) using Annexin II Antibody (HY-P87723) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/4000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
Background
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Function
Annexin II is a Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9. Binds to endosomes damaged by phagocytosis of particulate wear debris and participates in endosomal membrane stabilization, thereby limiting NLRP3 inflammasome activation. Required for endothelial cell surface plasmin generation and may support fibrinolytic surveillance and neoangiogenesis|(Microbial infection) Binds M.pneumoniae CARDS toxin, probably serves as one receptor for this pathogen. When ANXA2 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen[1][2][3].
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Subcellular Localization
Secreted, extracellular space, extracellular matrix, basement membrane,Melanosome
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Isoforms & Post-Translational Modification
P07355 has two isomers: P07355-1: 38604 Da (predicted); P07355-2: 40411 Da (predicted).
Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface丨ISGylated -
Subunit
Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36 (By similarity)
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SwissProt ID
Documentation
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