2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies
  5. CD3D Antibody (YA3639)(PBS only)

CD3D Antibody (YA3639)(PBS only)

製品番号: HY-P83942A
User Guide for Antibodies Technical Support

CD3D Antibody (YA3639) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to CD3D.

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容量 在庫状況
50 μL   お問い合わせ  
100 μL   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • 説明

製品説明

CD3D Antibody (YA3639) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to CD3D.
Host

Mouse
分子量
Predicted band size: 19 kDa;
Observed band size: 23 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Purified recombinant fragment of human CD3D (AA: 127-171) expressed in E. Coli.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:2000
FC
FC: Flow Cytometry
1:200-1:400
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
1:10000
純度 affinity purified. Conjugation Non-conjugated
Isotype IgG2b  
Appearance

Liquid
Formulation

Supplied in PBS, pH 7.4.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
輸送条件

Shipping with blue ice.
Background
Function：Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed:12215456)
Subcellular Localization：Cell membrane; Single-pass type I membrane protein
Expression：
Tissue_specificity:CD3D is mainly found on T lymphocytes and binds to its TCR-CD3 chaperone protein. CD3D is also present in fetal NK cells.
Isoforms & Post-Translational Modification：P04234 has 2 isomers: P04234-1: 18930 Da (predicted); P04234-2: 14484 Da (predicted).
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8
Subunit：The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta. Interacts with coreceptors CD4 and CD8 (PubMed:12215456, PubMed:1396954)
Synonyms
T3D; IMD19; CD3-DELTA
ドキュメンテーション
User Guide for Antibodies (1077 KB)

CD3D Antibody (YA3639)(PBS only) 関連分類

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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製品名:
CD3D Antibody (YA3639)(PBS only)
製品番号:
HY-P83942A
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